星形胶质细胞外泌体来源环状RNA对SCI后血脊髓屏障功能的影响及陇中消肿止痛合剂干预机制研究

脊髓损伤主要危害中枢神经系统功能，造成神经细胞及中枢神经系统的传导功能丧失，严重影响患者生存和生活质量。神经元功能不足是SCI的标志，目前医学界公认的科研方向及治疗方向主要围绕如何减少神经细胞的凋亡和CNS轴突的再生。然而，星形胶质细胞，小胶质细胞，周细胞，内皮细胞，雪旺细胞，成纤维细胞，脑膜细胞和其他胶质细胞的继发性损伤反应不仅增强了SCI损伤，而且还促进了内源性修复。神经胶质疤痕内和周围，细胞会沉积影响轴突生长的细胞外基质蛋白。目前间质干细胞分外泌体环状RNA通过信号通路调控椎间盘退变的髓核细胞凋亡，从而通过基因层面治疗椎间盘退变的髓核细胞凋亡，从而通过基因层面治疗椎间盘病变提供新的治疗思路和靶点。本研究也是从SCI损伤中提取关键细胞—AS，通过研究AS所分泌的外泌体是否通过干预血脊髓屏障通透性，以此缓解脊髓水肿调控神经细胞的凋亡及促进CNS轴突再生，以此寻找治疗SCI的靶点及思路。

Spinal cord injury mainly harms the function of central nervous system, resulting in the loss of nerve cells and conduction function of central nervous system, which seriously affects the survival and quality of life of patients.Inadequate neuronal function is a sign of SCI. Currently, the recognized research and treatment directions in the medical community mainly focus on how to reduce the apoptosis of nerve cells and the regeneration of CNS axons.However, the secondary injury response of astrocytes, microglia, pericytes, endothelial cells, schwann cells, fibroblasts, meningeal cells, and other glial cells not only enhanced SCI injury, but also promoted endogenous repair.In and around the glial scar, cells deposit extracellular matrix proteins that affect axon growth.At present, mesenchymal stem cells exosomal circrnas regulate the apoptosis of nucleus pulposus cells in disc degeneration through signaling pathway, so as to treat the apoptosis of nucleus pulposus cells in disc degeneration at the gene level, thus providing new therapeutic ideas and targets for treating disc diseases at the gene level.In this study, the key cell -- AS was extracted from SCI injury. By studying whether the exosomes secreted by AS can interfere with the permeability of the blood and spinal cord barrier, it can alleviate the spinal cord edema, regulate the apoptosis of nerve cells and promote the regeneration of CNS axons, so AS to find the target and train of thought for the treatment of SCI.