近年来，中医药辨证施治在治疗继发性脊髓损伤（SSCI）中体现出一定优势，为此我们以“补督脉、行气血”为法则，运用甘肃省中医院院内制剂-陇中消肿止痛合剂治疗SSCI，取得较好的临床疗效，并通过前期实验研究，证实其在SSCI中对脊髓的保护作用。本项目拟在前期研究基础上，进一步探讨陇中消肿止痛合剂对SSCI中血-脊髓屏障(BSCB)的保护作用及相关分子生物学机制，通过构建大鼠脊髓损伤模型及缺氧-复氧血-脊髓屏障细胞模型，从基因调控水平深入分析lncRNAMALAT1通过ERK/p38MAPK信号通路调控BSCB中AQP-4表达的机制，并阐明陇中消肿止痛合剂干预lncRNAMALAT1-ERK/p38MAPK-AQP-4信号轴的药效学机理。为中医“补督脉、行气血”法治疗SSCI提供科学依据，为陇中消肿止痛合剂防治SSCI的进一步推广应用奠定理论基础，为中医药防治SSCI提供新的策略思路及治疗靶点。

In recent years, syndrome differentiation and treatment of traditional Chinese medicine （TCM）have shown some advantages in the treatment of secondary spinal cord injury (SSCI). So we make "fill the Du meridian, Qi and blood" as the principle, using the hospital preparation - Longzhong Xiaozhongzhitong Mixture that is hospital preparation in Gansu provincial hospital of TCM in the treatment of SSCI, has good clinical curative effect. At the same time, the protective effect of it on the spinal cord in SSCI was confirmed by previous experiments. Based on previous studies, we will further explore the protective effect of Longzhong Xiaozhongzhitong Mixture on blood-spinal cord barrier (BSCB) in SSCI and related molecular biological mechanisms through the establishment of the rat model of spinal cord injury and the cell model of hypoxia reoxygenation BSCB, in-depth analysis of the mechanism of lncRNAMALAT1 regulating the expression of AQP-4 in BSCB through ERK/p38MAPK signaling pathway from the level of gene regulation. It will clarify the intervention efficacy of Longzhong Xiaozhongzhitong Mixture on lncRNAMALAT1-ERK/p38MAPK-AQP-4 signal axis mechanism, provide a scientific basis for "fill the Du meridian, Qi and blood" of TCM in the treatment of SSCI, lay a theoretical foundation for the further popularization and application of the prevention and treatment of SSCI in Longzhong Xiaozhongzhitong Mixture, and provide a new strategy and target for the prevention and treatment of SSCI in TCM.