FD-OCT联合CMR成像技术探索LncRNA NEAT1在动脉粥样硬化进程中的作用与机制

动脉粥样硬化（AS）是冠心病的病理基础，在AS进程中巨噬细胞数量增加和M1/M2型比例失衡是促使斑块不稳定的重要原因。因此，应用影像学技术定性定量监测巨噬细胞预防心血管事件的发生亟待解决。申请人前期国自然利用FD-OCT检测发现斑块稳定性与let-7b调节巨噬细胞自噬和极化密切相关。FD-OCT能定量检测巨噬细胞，但不能区分巨噬细胞亚型，而心脏核磁共振（CMR）分子成像技术能定性检测巨噬细胞，故本课题拟结合FD-OCT/CMR定性定量监测巨噬细胞评估斑块稳定性。预实验发现NEAT1在冠心病患者单核细胞中上调，可调控巨噬细胞自噬和极化。我们通过生物信息预测NEAT1可结合let-7b，推测NEAT1可能是AS进展中的关键分子靶点。本项目从临床出发，应用FD-OCT/CMR成像技术阐明NEAT1/Let-7b/TGF-βR1调控巨噬细胞自噬和极化的机制，为冠心病治疗提供新靶点和影像学支持。

Atherosclerosis (AS) is the pathological basis of coronary heart disease. The increase in the number of macrophages and the imbalance of M1 / M2 type ratio in AS process are important reasons for plaque instability. Therefore, the application of imaging technology to qualitative and quantitative monitoring of macrophages to prevent cardiovascular events needs to be solved. The applicant's previous National Natural Science Foundation of China used FD-OCT to find that plaque stability is closely related to let-7b regulating macrophage autophagy and polarization. FD-OCT can quantitatively detect macrophages, but it cannot distinguish macrophage subtypes. Cardiac magnetic resonance (CMR) molecular imaging technology can qualitatively detect macrophages. Therefore, this subject intends to combine FD-OCT and CMR to qualitatively and quantitatively monitor macrophages to assess plaque stability.Pre-experiments found that NEAT1 is up-regulated in monocytes of patients with coronary heart disease and can regulate autophagy and polarization of macrophages. We predict from biological information that NEAT1 can be combined with let-7b. It is speculated that NEAT1 may be a key molecular target in the progress of AS. This project is clinically based and uses FD-OCT / CMR imaging technology to elucidate the mechanism by which NEAT1 / Let-7b / TGF-βR1 regulates macrophage autophagy and polarization, providing new targets and imaging support for coronary heart disease treatment.