联合FD-OCT评估let-7b调控巨噬细胞自噬影响动脉粥样硬化斑块稳定性机制的研究

动脉粥样硬化(AS)是冠心病及脑卒中发生的主要病因，在现有他汀等药物抗AS治疗下仍有较大残余风险。我们中心应用频域光学相干断层成像技术(FD-OCT)在体连续识别冠心病患者斑块特征的研究提示，巨噬细胞仍是AS进展的独立预测因素。然而目前易损斑块内巨噬细胞浸润的定性及定量的评估方法仍需进一步完善。本课题通过联合应用FD-OCT及血管内超声(IVUS)在体连续评估斑块特征，首次通过OCT-IVT分析软件，选取目标区域应用规范化标准差(NSD)算法精确计算出易损斑块中巨噬细胞含量，并通过病理学及分子生物学实验阐明其准确性与实用性，通过干预巨噬细胞的功能状态稳定AS斑块，为治疗AS的新靶点和方向提供新的影像学依据。

Atherosclerosis (AS) is the main cause of coronary heart disease and stroke. There is still a large residual risk is existing in the base of statin and other anti-AS treatments. Our studies using optical coherence tomography (OCT) in vivo to continuous identify plaque features in patients with coronary artery disease suggest that macrophages are an independent predictor in AS progression. However, there is still lack of ideal imaging techniques for the quantitative and qualitative assessment of macrophage in vulnerable plaques in vivo. Our research will combine FD-OCT with IVUS to consecutive assess plaque features in vivo, the target region was selected to calculate the macrophage content of vulnerable plaques by using the variance standard deviation (NSD) through the OCT-IVT analysis software, and to clarify its accuracy and practicality through pathology and molecular biology experiments. We stabilize the AS plaque by interfering with the functional status of macrophages and provide a new imaging basis for the new target and direction to AS therapy.