新孢子虫诱导的中性粒细胞胞外诱捕网对母牛胎盘滋养上皮的损伤机制

批准号:
32002309
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
魏正凯
依托单位:
学科分类:
兽医寄生虫学
结题年份:
2023
批准年份:
2020
项目状态:
已结题
项目参与者:
魏正凯
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中文摘要
本项目针对新孢子虫感染母牛导致流产、死胎等繁殖障碍性疾病,目前还缺乏有效的防治手段这一生产实践问题,从先天免疫学理论中有关中性粒细胞胞外诱捕网(NETs)在对抗病原的同时,也会造成宿主自身组织免疫损伤入手,在前期体外证实NETs能够捕获、杀灭新孢子虫的工作基础上,提出胎盘组织NETs的释放,可能是新孢子虫感染母牛导致流产、死胎等繁殖障碍的关键致病环节。项目拟建立母牛新孢子虫感染模型,以胎盘组织NETs为研究对象,利用扫描电镜、共聚焦等技术,首先观察胎盘绒毛间隙NETs形态特征;其次评价NETs对胎盘组织的损伤情况,分析哪些NETs组分损伤胎盘滋养上皮;最后揭示NETs损伤胎盘滋养上皮的作用机制。项目旨在阐明新孢子虫病母牛流产、死胎繁殖障碍过程中,有关胎盘滋养上皮免疫损伤的详细机制,从而为开发有效防治新孢子虫病的疫苗和药物提供理论依据和参考,同时对其他顶复门原虫病防治研究也具有借鉴意义。
英文摘要
The project aimed at the problems of lack effective methods for prevention and treatment with cows-infected with Neospora caninum (N. caninum) which leading to reproductive disorders including abortion and stillbirth in productive practice. Starting with the innate immunology related theory that neutrophil extracellular NETs (NETs) can simultaneously result in the immunological damage of host tissue while fighting against infection. On the basis of previous researches that NETs could capture and kill N. caninum, it is proposed that N. caninum-triggered NETs release in placental tissue could be the key point in the process of cow reproductive disorders including abortion and stillbirth. In this project, the cow model of N. caninum infection was established to study NETs release in placenta tissue. Scanning electron microscopy, confocal microscopy and other technology were selected to firstly observe the structural characteristics of NETs in placenta inter-villous space. Secondly, the effects of NETs on placenta tissue damage were evaluated, and which components of NETs resulting in placental trophoblastic epithelium damage were further analyzed. Finally, the mechanism underlying NETs-induced placental trophoblastic epithelium damage was revealed. Taken together, this project was performed to clarify the detailed mechanism involving in placental trophoblastic epithelium damage in the process of N. caninum -induced reproductive obstacle disease of cows, which would provide theoretical basis and experimental reference for developing effective vaccines and drugs against neosporosis, as well as play a reference significance role in researches on the prevention and treatment with other protozoosis.
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DOI:10.1007/s00204-022-03357-4
发表时间:2022-08
期刊:Archives of Toxicology
影响因子:6.1
作者:Liqiang Jiang;W. Liu;Jingnan Xu;Xinxin Gao;Haiguang Zhao;Shurou Li;Wenlong Huang;Zhengtao Yang;Zhengkai Wei
通讯作者:Liqiang Jiang;W. Liu;Jingnan Xu;Xinxin Gao;Haiguang Zhao;Shurou Li;Wenlong Huang;Zhengtao Yang;Zhengkai Wei
DOI:--
发表时间:2022
期刊:Archives of Toxicology
影响因子:--
作者:Liqiang Jiang;Shuangqiu Li;Di Wu;Aimin Jiang;Ziyi Liu;Xingyi Zhu;Yong Zhang;Jingnan Xu;Xinxin Gao;Wei Liu;Zhengtao Yang;Zhengkai Wei
通讯作者:Zhengkai Wei
DOI:10.1021/acs.jafc.1c05371
发表时间:2021-10
期刊:Journal of agricultural and food chemistry
影响因子:6.1
作者:Wei Liu;Di Wu;Shuangqiu Li;Jingnan Xu;Peixuan Li;Aimin Jiang;Yong Zhang;Ziyi Liu;Liqiang J
通讯作者:Wei Liu;Di Wu;Shuangqiu Li;Jingnan Xu;Peixuan Li;Aimin Jiang;Yong Zhang;Ziyi Liu;Liqiang J
DOI:10.1016/j.jinorgbio.2022.111838
发表时间:2022-04
期刊:Journal of inorganic biochemistry
影响因子:3.9
作者:W. Liu;Wenlong Huang;Shurou Li;Haiguang Zhao;Liqiang Jiang;Jingnan Xu;Xinxin Gao;Zhengtao Yang;Zhengkai Wei
通讯作者:W. Liu;Wenlong Huang;Shurou Li;Haiguang Zhao;Liqiang Jiang;Jingnan Xu;Xinxin Gao;Zhengtao Yang;Zhengkai Wei
Quercetin alleviates gliotoxin-induced duckling tissue injury by inhibiting oxidative stress, inflammation and increasing heterophil extracellular traps release.
槲皮素通过抑制氧化应激、炎症和增加异嗜细胞外捕获物的释放来减轻胶质毒素引起的小鸭组织损伤。
DOI:--
发表时间:2023
期刊:Food and Chemical Toxicology
影响因子:4.3
作者:Qinqin Jin;Mei;Zhang Jin;Yuqian Jiang;H. Hong;Y. Qian;Wei Liu;Xinxin Gao;Liqiang Jiang;Jingnan Xu;Quan;Zhengkai Wei
通讯作者:Zhengkai Wei
新孢子虫免疫逃逸犬中性粒细胞胞外诱捕网的分子机制
- 批准号:n/a
- 项目类别:省市级项目
- 资助金额:10.0万元
- 批准年份:2022
- 负责人:魏正凯
- 依托单位:
国内基金
海外基金
