保加利亚乳杆菌是酸奶发酵剂主体菌种之一，其高生物量是开发高效直投式发酵剂的关键。目前研究发现采用代谢调控、营养充足时菌体增殖能力仍会下降。菌体增殖与细胞分裂能力及速度密切相关，而细胞分裂骨架的形成及调控决定着分裂与否。本课题以高密度培养中为中和菌体产生的酸而引起的盐胁迫为条件，利用分子定量、荧光标记等技术，探索保加利亚乳杆菌分裂动态变化模型，找到分裂受阻临界点；在此基础上，研究细胞分裂关键步骤变化，确定盐胁迫对细胞分裂骨架形成的影响；利用转录组、蛋白组及生物信息学技术比较菌体分裂受阻时相关基因及蛋白表达差异，确定影响分裂骨架形成的关键蛋白及基因，揭示乳杆菌分裂机制及途径；利用细胞信号系统、蛋白互作网络、基因沉默等技术，通过关键基因/蛋白对分裂骨架进行调控，构建关键基因/蛋白的“转录因子-蛋白互作”调控体系，揭示细胞分裂骨架调控机制，对阐明菌体分裂增殖机理及高效生产乳酸菌发酵剂具有重要意义。

L. bulgaricus is the main component of lactic acid starter. Its high biomass is an important way to achieve efficient and low-cost production. At present, although grown in a suitable situation and abundant nutrition, the proliferation of bacteria is still stagnated. The proliferation of bacteria is closely related to the ability of cell division. Furthermore, the formation of cell division skeleton and its regulation are crucial to cell division ability. In this paper, a dynamic model of L. bulgaricus cell division hindrances was established and the critical point of division was found by using molecular quantification and fluorescence labeling techniques under the condition of salt stress. Based on which, we investigated cell division processes and analyzes the effect of salt stress on cell division and cytoskeleton formation. Transcriptomics, proteomics and bioinformatics technology will be applied to show the differences of gene expression and proteins, to find out the key enzymes and genes which affects bacteria division and elucidate the pathway and mechanism of L. bulgaricus cell division. Thereby, the key genes/proteins will be used to explore the regulation mechanism of L. bulgaricus by using signal systems, protein network interaction technologies. Based on which, "transcription factor-protein interaction" regulation network for the key gene/protein will be constructed. And these will supply a new method and idea for production high cell density cultivation of L. bulgaricus.