AGAMOUS-LIKE FLOWER分子调控网络在豆科植物花发育中的功能研究

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中文摘要
花器官的确立被ABC模型所调控,A类基因调控萼片的形成,A和B类调控花瓣,B和C类调控雄蕊,C类调控心皮。上述ABC花发育模型基于拟南芥和金鱼草建立。蒺藜苜蓿是豆科模式植物,花器官发育与拟南芥有很大差异。为了研究豆科模式植物蒺藜苜蓿的花发育机制,我们从蒺藜苜蓿Tnt1逆转录转座子突变体库中,筛选得到aglf突变体。其表型为雄蕊和心皮转换为重叠花瓣和萼片,这是继C类基因AGAMOUS之后首次鉴定到的强表型单拷贝新C类功能基因。我们以AGLF为起点,鉴定了其下游的RAGLF和IRAGLF,对其表型进行了分析,并对AGLF上游的PBAGLF展开了初步的筛选。在本项目中,我们拟对这些突变体和相关基因开展遗传分析和功能研究,并对这些基因与ABC基因和AGLF基因的调控关系开展研究。阐明AGLF的分子调控网络为建立豆科植物花发育基因模型奠定基础,对植物花器官发育,特别是豆科植物花器官发育具有重要意义。
英文摘要
Floral identity is controlled by the well-known ABC model, in which Class A genes control sepal and petal development, Class B genes control petal and stamen development and Class C gene(s) control stamen and carpel development. The ABC model was summarized from genetic studies on the model plants Arabidopsis thaliana and Antirrhinum majus. As the model legume species, Medicago truncatula develops bilaterally symmetric flowers different from those of Arabidopsis. To understand the mechanism underlying flower development in M. truncatula, in a previous work we characterized a novel transcription factor from the Tnt1 retrotransposon mutant library, AGAMOUS-Like Flower (AGLF), that provides C function in floral identity. Here, we propose to study the molecular function of AGLF by exploring its position during flower development. Our preliminary results have identified RAGLF and IRAGLF downstream of AGLF from transcriptome, ChIP (chromatin immunoprecipitation) sequencing data and yeast-2-hybrid. On the second hand, PBAGLF was identified upstream of AGLF by yeast-1-hybrid. Elaborating RAGLF's, IRAGLF's and PBAGLF's regulatory roles and their relationships with other ABC genes and AGLF will not only dissect the regulatory network of floral identity in M. truncatula, but bring new perspectives to the flower development in legume species.
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REVOLUTA基因在豆科模式植物蒺藜苜蓿复叶发育中的功能研究
- 批准号:31400262
- 项目类别:青年科学基金项目
- 资助金额:24.0万元
- 批准年份:2014
- 负责人:赵阳
- 依托单位:
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海外基金
