急性肝衰竭是肝病防治领域中的重点与难点问题之一，单纯西医治疗效果不理想。研究表明肝细胞焦亡是导致急性肝衰竭进程加速的重要因素。中医经方茵陈蒿汤联合西医治疗肝衰竭已显示确切临床疗效。课题组前期研究发现茵陈蒿汤可协同脐带间充质干细胞（ucMSCs）调控急性肝衰竭肝细胞焦亡，且miR-23b的表达在茵陈蒿汤干预的ucMSCs-exosomes中明显增高。本项目拟以肝细胞焦亡为切入点，提出茵陈蒿汤可通过促进ucMSCs-exosomes中miR-23b含量的增高而抑制肝细胞焦亡和肝衰竭。因此，本项目拟在前期研究基础上建立急性肝衰体内外模型，探索茵陈蒿汤调控ucMSCs-exosomes中miR-23b的表达及miR-23b抑制肝细胞焦亡的作用途径及具体分子机制，进一步阐明茵陈蒿汤协同ucMSCs-exosomes调控肝衰的分子机理，为两者在肝衰中的联用提供理论基础和实验依据。

Acute liver failure(ALF) is one of the important and difficult problems in the liver disease, and the therapeutic effect of simple western medicine is poor. The pyroptosis of hepatocytes is the main factor in accelerating the process of ALF. The combination of tranditional Chinese medicine prescription of Yinchenhao Decoction and western medicine in the treatment of liver failure has showed exact clinical curative effect. Our earlier studies have showed synergistic therapeutic effect of Yinchenhao decoction and umbilical cord mesenchymal stem cells (ucMSCs) on acute liver failure, and miR-23b expression was significantly increased in ucMSCs-exosomes treated with Yinchenhao Decoction. This project will investigate the mechanism of Yinchenhao decoction in regulating the expression of miR-23b in ucMSCs-exosomes and the mechanism of miR-23b in inhibiting the pyroptosis of hepatocytes. The project will explore the mechanism of the synergistic therapeutic effect of Yinchenhao decoction and ucMSCs on ALF from a new perspective and provide therotical and experimental evidence for the treatment of ALF with Traditional Chinese Medicine and Western medicine.