开发避孕节育新方法是避免非意愿怀孕发生，保护妇女生殖健康的重要措施。组合不同抗原、不同表位，构建多表位肽疫苗可以提高避孕的有效性和安全性，是免疫避孕疫苗研究的新方向。精子膜蛋白Izumo1、ADAM2和ADAM3都是受精过程中的关键分子，其基因缺陷的雄性小鼠都是不育的。而且这三个蛋白密切相关，相互调控。ADAM2和ADAM3呈复合物形式存在于小鼠精子膜，调控精子与卵透明带的结合。ADAM2在Izumo1与卵膜CD9分子的结合过程中起重要的桥梁作用，介导两者的结合，促使精-卵融合的顺利进行。因此，本项目将Izumo1、ADAM2和ADAM3作为研制避孕疫苗的靶标，采用“改良生物合成肽法”筛选其全部BCE，然后将这三个靶蛋白中保守性高，特异性强、抗生育作用显著的BCE组合在一起，构建多表位肽疫苗，以产生更高效、安全、可逆的避孕效果。

The development of new and suitable contraceptive methods is an important measure to avoid the occurance of unwanted pregnancy and to protect women's reproductive health. The combination of several linear BCEs from different antigens related-infertility for constructing chimeric peptide vaccine will improve the contraceptive effect, which is the new direction of the contraceptive vaccine research. Izumo1, ADAM2 and ADAM3 are all sperm membrane proteins and play important roles in the sperm-egg binding and fusion during fertilization. Male mice lacking Izumo1, Adam2 or Adam3 are all infertile. ADAM2-ADAM3 complex in sperm has a potential role in sperm binding to the egg zona pellucida. ADAM2 mediate the interaction between two essential factors CD9 and IZUMO1. In this study, all BCEs of Izumo1, ADAM2 and ADAM3 will be identified by improved biosynthetic peptides method. Then we will choose the BCEs, which are highly conserved, tissue specific and have significant antifertility effect, to construct multi-epitope peptide vaccines. The vaccines may produce more efficient, safer and more reversible contraceptive effect, laying the foundation for 100% contraception.