人和动物对膳食脂肪的偏好导致高脂饮食摄入增加、肥胖和相关的代谢性疾病发生率升高。脂肪味觉在脂肪偏好的形成中起关键作用。中医理论认为“心藏神，在志为喜”、“心开窍于舌”，因此，舌通过影响心神，进而改变喜好，在饮食偏好中发挥重要作用。CD36是舌味觉感受细胞感受脂肪酸的主要受体，其表达及受体后信号转导与脂肪偏好密切相关。前期研究表明，中药复方活血降糖饮能有效降低肥胖大鼠体重，改善糖脂代谢，改善脂肪偏好，但作用机制不明。本项目以c57bl/6小鼠为研究对象，通过活血降糖饮干预肥胖小鼠，通过行为学试验观察小鼠对脂肪偏好的变化，并分析CD36的表达、质膜定位、泛素化降解以及胞内相关信号，胞内神经递质合成与释放，以及其他组织CD36及相关蛋白的表达，从“舌-心(神)-欲”的角度探讨活血降糖饮早期干预对不同代谢状态小鼠脂肪偏好的影响和可能的作用机制。

The fat preference of both human being and rodents leads to high fat diet, and consecutively, the increase of obesity and related metabolic diseases. However, fat oro-sensory plays an important role in the formation of fat preference. In Traditional Chinese Medicine theory, it is recognized that “the tongue is the orifice to the heart”, “happy and loving are the spirit of heart”, and that “spirit stored in the heart”. So, the tongue affects preference through regulating heart spirit, and is of great significance in diet preference. CD36 is the major fat acid receptor on taste bud cells on tongue, the change of whose expression and signal transduction in cell influences fat preference gravely. In our previous studies, we have proved that the Chinese herbal compound Huo-xue-jiang-tang decoction decrease the weigh; regulate glucose and lipid metabolism of rats effectively; and modulates fat preference on rats, however, the mechanism is to be elucidated. In this research, c57bl/6 mice are recruited to investigate the effect of Huo-xue-jiang-tang decoction on fat preference of obese mice; examine the expression, dislocation on plasmid membrane, Ubiquitination and degradation, and signal transduction of CD36; and CD36 expression in other organs, so as to explain the effect and molecular mechanism of Huo-xue-jiang-tang decoction of fat preference of mice through “the tongue is the orifice to the heart” theory.