酶促反应是生物转化的核心，克服底物扩散缓慢、产物反馈抑制及催化剂回收困难是生物化工过程发展的挑战。本项目拟构建双水相液滴微流平台进行强化酶促反应过程的机理研究：（1）通过设计微流控装置、双水相体系以及制备共聚物表面活性剂，获得一个选择性透过的全水相液滴微流稳定界面，将大分子酶关在液滴微反应器内，而允许小分子物质跨界面定向传递。（2）通过调变流动参数和界面因素，揭示目标溶质跨全水相液滴界面的传质过程规律及强化机制。（3）以脲酶和青霉素酰化酶催化为模型反应，阐明酶存在下的底物/产物传递特性，并依靠特殊相界面，从快速传递，高效混合和反应分离的协同耦合入手，探索提高酶促反应过程整体效率的方法和机理。研究结果将为实现绿色、高效的生物反应分离过程提供新途径，为双水相液滴微流体系促进生物化工过程强化和化工系统小型化奠定理论基础。

Enzymatic catalysis is one of the critical issues in biotransformation processes, where the problems of slow transfer of substrates, feedback inhibition by products, and poor reuse of biocatalysts are still challenges towards the development of biochemical engineering. In this project, droplet microfluidics with aqueous two-phase systems (APTSs) is proposed as novel bioreactors for the intensification of enzymatic catalysis. At first, a stable microdroplet water/water (W/W) interface is obtained, bases on the employment of proper APTSs, conjugate emulsifiers and designed microchannels. Such W/W interfaces with permselective property allow small molecules to transport across, whereas compartmentalize the enzyme molecules in the microdroplets. Then mechanism study on mass transfer enhancement across the fluid all-aqueous interfaces will performed by means of interface construction as well as fluid manipulation. Using urease and penicillinase catalysis as model reactions, transport characteristics of substrates and products in the presence of enzymes are especially demonstrated. Based on the unique interfaces, rapid microtransfort, efficient micromixing and simultaneous reaction-separation are synergistic coupling, with the aim of researching and developing enzyme-catalyzed process intensification on micrometer scale. This project will not only open avenues for green and effective bioreaction/separation processes, but also provides promising methods for process intensification of biochemical engineering and for miniaturization of chemical engineering systems.