改性坡缕石负载环糊精合成及其作为环境水体中抗生素固相萃取吸附剂研究

采用不同硅烷偶联剂对坡缕石进行改性，再分别与母体环糊精(α－CD，β－CD，γ－CD)或取代环糊精(HP－CD，甲基化CD，乙基化CD等)反应，生成新型的改性坡缕石负载环糊精。通过元素分析及谱学方法对改性坡缕石负载环糊精的组成、结构等进行表征。将这种改性坡缕石负载环糊精作为固相萃取吸附剂，应用于环境水体(地表水、地下水、污水处理厂进出口水)中抗生素(如，大环内酯类抗生素、磺胺类抗生素、氟喹诺酮类抗生素及其它抗生素等)的富集、预处理中，探讨所使用的改性剂类型、固相萃取柱柱长、上样速度、样品溶液pH及盐度等因素对不同类型抗生素萃取效率的影响，优选出适合不同类型抗生素的最佳固相萃取条件和最佳固相萃取吸附剂。然后，采用色谱(或色－质联)方法对萃取物中多种抗生素进行同时测定(定性、定量分析)，探讨色谱柱类型、流动相类型及配比、检测条件等对方法准确性、重现性、回收率等的影响。

Silane coupling agents are used on the modification of palygorskite, the resulted products will further react with parent cyclodextrins (α-CD, β-CD and γ-CD) and modified cyclodextrins (HP-CD, methyl-CD, ethyl-CD), respectively, to give new types of modified palygorskite bearing cyclodextrins. Elemental analysis, IR, XRD, and DTA-DGT will be used to characterize the compositions and structure of the products synthesized. The modified palygorskite bearing cyclodextrins will be used as extraction agent for solid phase extraction of antibiotics in environmental water (surface water, groundwater, and sewage processing factory import and export water, etc). Factors on extraction efficiency, eg. the type and composition of solid extraction agent, diameter of solid extraction column, speed of sampling, concentration and pH of the water samples loaded, will be investigated. The concentrated solutions obtained from solid-phase extraction under best extraction conditions will be separated and characterized by using chromatography (or chromatography-MS) for qualitative and quantitative analysis. The accuracy, recovery, and reproducibility of the whole extraction and analysis process will be evaluated.