本研究基于2型糖尿病“壮火食气”病机理论，提出清热益气治法的黄连人参对药可以通过调节胰岛β细胞内PDX-1基因甲基化，影响FoxO1、PDX-1的表达，进一步调控β细胞的数量和功能假说。在既往实验研究基础上，采用自发性2型糖尿病db/db小鼠建立动物模型，采用两因素析因设计，研究黄连人参对药通过调节PDX-1基因甲基化，影响PDX-1、FoxO1、Ngn3、胰岛素基因的表达，促进β细胞分化再生的微观机制，探索其纠正代谢应激，抑制β细胞去分化，促进胰岛祖细胞样β细胞再生的作用途径，进一步揭示2型糖尿病清热益气治法的机理，为中药促进β细胞损伤修复，改善β细胞功能提供新的依据和思路。

The study is based on the TCM theory of ‘excessive fire consuming qi’ of type 2diabetes. We proposed the hypothesis that berberine and ginseng in forms of clearing heat and supplying qi method can regulate the methylation of PDX-1 in vivo and thus regulate the number and function of islet β cell. we use spontaneous type 2 diabetes db / db mice animal model, set up two-factor factorial design, and investigate the regulation of ginseng berberine on the genetic methylation of PDX-1,and influence on the expression of FoxO1,PDX-1, insulin, Ngn3, in order to explore the mechanism of β cell differentiation and regeneration, the pathways of inhibiting β cells differentiation and promoting progenitor cell-like β cell regeneration, and the mechanism of the clearing heat and supplying qi method on the type 2 diabetes treatment as well. Thereby, provide new evidence and new ideas of Chinese medicine for promoting β-cell damage repair and improving β-cell function.