Omega-3多不饱和脂肪酸（n-3 PUFA）具有抗乳腺癌作用，但其机制并不明确。n-3 PUFA 经过体内复杂的代谢网络被代谢成上百种类二十烷酸产物，这些产物具有丰富的生物学功能，是n-3 PUFA发挥作用的重要介质，其中某些产物的功能可能与肿瘤有关。n-3 PUFA可能通过对类二十烷酸代谢网络的调控，从多个角度行使抗肿瘤功能。本项目将借助于已建立的类二十烷酸代谢组学方法，以乳腺癌患者及乳腺癌裸鼠模型，研究患乳腺癌状态下类二十烷酸代谢网络的系统性改变，发现n-3 PUFA如何通过影响类二十烷酸代谢网络起作用，筛选起重要调控作用的类二十烷酸分子靶点，并在细胞模型中研究这些分子的功能，并阐述其具体作用机制。以类二十烷酸代谢产物为靶点，可能寻找到效果更明显，作用方式更特异的抗肿瘤分子。

Omega-3 polyunsaturated fatty acids (n-3 PUFA) have some effect against breast cancer, but its mechanism is not clear. n-3 PUFA is onverted to hundreds of eicosanoids products via the complicated metabolic network in mammalian cell. Eicosanoids may be the important functional molecules mediating n-3 PUFA’s effects, and some eicosanoids’ function may be related to tumor. n-3 PUFA’s protective effects on tumor could be through the shift of eicosanoid metabolic network. In this project, we will study the systematic change of eicosanoid profile in patients with breast cancer and breast cancer model via the eicosanoid metabolomics method. We will prove the hypothesis that the myocardial infarction protective effects of n-3 PUFA are through eicosanoid metabolic network, and identify the eicosanoid targets with protective function. The aim of this project is to discover anti-tumor eicosanoid targets with more potent effects and specific mechanism.