线粒体网络重建在猪前脂肪细胞分化及脂肪沉积中的作用研究

调控猪脂肪细胞分化能够有效提高胴体瘦肉率和改善猪肉品质。线粒体网络（数量、形态、分布）对维持脂肪细胞功能至关重要，且线粒体网络状态的转变（线粒体网络重建）是决定脂肪细胞分化效率的关键因素。研究发现线粒体数目在猪脂肪细胞分化成熟的过程中呈动态变化，但猪脂肪细胞线粒体网络重建的动力学过程（融合、分裂与自噬）尚不清楚，因此申请人拟从线粒体网络的全新视角解析猪脂肪细胞的成熟过程。本项目拟通过分子检测和免疫电镜观察，阐明线粒体在猪前脂肪细胞分化过程中的动态变化过程，包括线粒体的数量、形态、分布、耗氧量和线粒体膜电位；分析猪前脂肪细胞分化及中外猪种成熟脂肪组织线粒体分裂融合和线粒体自噬蛋白的表达，确定分化过程线粒体分裂融合和自噬发生的“高峰期”；给予线粒体融合分裂和线粒体自噬相关的激活和抑制分子，研究其对猪前脂肪细胞分化成熟的影响，为探讨新的猪脂肪细胞分化和脂肪沉积机制提供理论基础。

Regulation of porcine preadipocyte differentiation can effectively improve carcass lean and the pork quality. Mitochondrial network (including number, morphology, and distribution) is essential for the maintenance of adipocyte function. And the transformation of mitochondrial network status (mitochondrial network reconstruction) is a key factor in determining the efficiency of adipocyte differentiation. Research reveales that the number of mitochondria in porcine adipocyte differentiation and maturation is dynamically changed. However, the dynamic process of porcine adipocyte mitochondria reconstruction (including fusion, fission, and autophagy) is still unclear. Therefore, we aim to analyze the maturation process of porcine adipocyte from a new perspective of mitochondrial network. To this end, we plan to use molecular detection and immune electron microscopy to determine the dynamic changes during pig preadipocyte differentiation, including the number, morphology, distribution, oxygen consumption of mitochondria and mitochondrial membrane potential. Analysis of the expression of fission-, fusion-, and autophagy-related proteins in the progress of pig preadipocyte differentiation and the adipose tissue of different pig breeds, to identify the expression "peaks" of related-proteins. The associated activator or inhibitor of the mitochondrial fusion, fission and mitophagy is added in medium to discover the effect on the process of pig preadipocyte maturation. This study would provide a new theoretical basis for the mechanisms of porcine adipocyte differentiation and fat deposition.