家蚕质型多角体病毒(BmCPV)基因组由10个dsRNA节段(S1-S10)组成，每个节段只编码一个蛋白基因。我们的前期研究发现BmCPV基因组的多个节段（S10，S5，S1）可能还编码潜在的小开放读码框(sORFs)，为探明潜在sORFs的功能，本项目在已筛选出BmCPV易感细胞系及建立的基于BmCPV dsRNA cDNA质粒的反向遗传学系统基础上，利用起始密码子突变明确sORFs的翻译起始，研究潜在的sORFs缺失病毒和sORFs修复病毒对细胞的病理变化、病毒增殖复制、子代病毒的感染性、病毒粒子的包装等影响，以及sORFs过表达对细胞凋亡、细胞周期和免疫通路等影响，拟从sORFs缺失和拯救病毒以及sORFs过表达对病毒和细胞的影响来探讨BmCPV基因组编码的sORFs的潜在功能。本项目不仅能明确BmCPV基因组编码的sORFs的功能，还可为认识BmCPV基因组信息提供全新的线索。

Bombyx mori cypovirus (BmCPV) genome consisting 10 dsRNA segments (S1-S10), and each segment encoded with single gene. Our previous study indicated that potential small open reading frames (sORFs) were respectively found in the BmCPV genomic segment S1, S5 and S10. To discover the potential roles of these sORFs, we have screened and obtained the BmCPV susceptible cells from the silkworm, and established the BmCPV reversed genetic system based on the recombinant plasmids containing full-length genomic dsRNA segment cDNA. The initial of sORFs translation was identified using mutations of the initiation codon based on the reverse genetic system. On this basis, the cytopathology, viral replication and multiplication, infectivity of progeny virus and virus particle package could be studied with the mutation and recovery of the sORFs from the dsRNA segments based on the reverse genetic system. Subsequently, cell apoptosis, cell cycle and immune pathways will be studied with overexpression of sORFs in the cultured cells. We plan to disclose the role of these sORFs in the genome of BmCPV from the influences to the virus and cells by the mutation and recovery of sORFs and its overexpression. This project will not only disclose the functions of sORFs in each dsRNA segment, study also provides the novel clues to better understand the genetic information of the BmCPV genome.