癌症治疗困难的一个重要原因是癌症成因的复杂性和异质性。对癌症相关基因突变的深入研究是人类战胜癌症必要的一环。用Sleeping Beauty（SB）等转座子为工具配合高通量测序筛选癌症相关基因已被证明是一种高效可靠的方法。我们已成功完成了用小鼠骨髓永生细胞筛选白血病相关基因的研究（Cancer Research, 2016）。在此基础上，我们拟开展以人支气管永生细胞为模型，用SB转座子为工具筛选肺腺癌相关基因的研究。前期通过半固体培养基克隆形成实验和二代测序我们找到了675个候选基因，并对其中一些进行了验证和机制方面的研究。初步结果显示我们的模型很好地模拟了肺腺癌的发生。我们拟对结果进行更系统深入的生物信息学分析，并对FUT4等基因的功能展开深入研究，同时开展肺癌抗药性相关基因的转座子筛选实验。肺癌是我国现阶段发病和死亡最多的癌症，我们希望通过本研究能够为肺癌治疗提供新的思路和靶点。

An important reason for the difficulty of cancer treatment is the complexity and heterogeneity of the causes of cancer. Comprehesive investigations on the cancer-related gene mutations is an essential part of fighting against cancers. Screening cancer-related genes with sleeping Beauty (SB) transposon and high-throughput sequencing has been proved to be a highly effective and reliable approach. We have successfully completed the screen of leukemia-related genes using BaF3, a mouse bone marrow immortalized cell line (cancer research, 2016). Based on this, we began to explore lung adenocarcinoma-related genes in HBEC, a human bronchial immortalized cell line, using SB transposon. In the early stage, we identified 675 candidate genes by clone formation experiments in semi-solid medium and Next-Generation Sequencing.The function of some of the candidate genes were then validated. Preliminary results suggest that our model successfully simulates the lung adenocarcinoma genesis. We then plan to conduct a more systematic and in-depth bioinformatics analysis of the results, and further study the functions of FUT4 and other genes, and carry out a screening experiment on drug-resistance associated genes in lung cancer. Currently, lung cancer is the most morbidity and mortality cancer in our country, we hope that this project will provide new ideas and targets for lung cancer therapy .