出血性中风（ICH）起病急，无有效治疗药物。研究证实ICH时的脑、肠损伤具有相关性。安宫牛黄丸（AGNH）为治疗ICH及昏迷的要药。现有研究只关注了AGNH治疗效应的“脑”作用，却忽视了其可能具有的“脑”外（如肠）作用途径。我们新近发现AGNH能保护ICH模型小鼠肠粘膜屏障，降低血LPS及脑组织炎症，提示AGNH治疗ICH的机制可能与其调节肠-脑通路作用有关。本课题结合小鼠脑出血和体外膜屏障（血脑与肠粘膜屏障）模型，以Garcia评分和脑电图等观察中风后不同时程神经功能状态，同时检测膜屏障、脑内外神经与炎症介质等的变化，明确AGNH神经保护作用的量效、时效关系，以及与膜屏障的关系和分子机制；进而通过干预交感、副交感神经，调节血LPS、血氨，检测脑、肠介质及肠菌易位等，明确AGNH改善ICH神经功能的肠-脑交互作用机制，为传统名方临床应用提供实验依据，为ICH的治疗提供新思路。

Hemorrhagic stroke, i.e. intracerebral hemorrhage (ICH), is very acute and has no effective treatment. Previous study confirmed there exist correlation between brain and intestinal injury after ICH. Angong Niuhuang Pill (AGNH) is a famous traditional Chinese medicine for ICH treatment. The current researches about the effects of AGNH on ICH pay only attention to brain instead of external cerebral pathway including gut. The results from our study showed that AGNH could protect gut barrier in ICH mice. AGNH could reduce the levels of blood LPS and inflammatory mediators. These findings indicated that the protective effects of AGNH on ICH may be related to regulating the gut-brain interaction. The present project will use ICH mice model and membrane barrier model in vitro to investigate the effects of AGNH on the impairments of blood-brain barrier (BBB) and brain-gut barrier. Garcia score and electroencephalogram were used to observe the neurological function after bleeding in different time points, and the changes of membrane barrier, the neurotransmitters and the inflammatory mediators will be evaluated at the same time, so as to elucidate the dose-effect relationship and time-effect relationship of AGNH. Then, the effects of AGNH on gut-brain interaction will be investigated by stimulating or blocking the sympathetic and parasympathetic nerve, regulating the levels of LPS and ammonia in blood, checking bacterial translocation with 16S rRNA sequencing detection. The present study aimed to elucidate the characteristics and mechanisms of AGNH attenuating the ICH injury by regulating gut-brain interaction. This project will provide a theoretical basis for traditional famous prescription (e.g. AGNH) being used to treat ICH. And it also supplies a new idea for researching and developing a strategy of treatment of ICH.