气孔是分布在所有陆地植物地上部表皮中调控植物与外界气体交换的重要细胞结构，是由原表皮细胞经过一系列不对称分裂和一次对称分裂，经历三种前体细胞形式所形成。已知拟南芥唯一的A型细胞周期蛋白依赖激酶CDKA;1是调控细胞分裂和分化的重要因子。我们利用花粉互补技术所获得的cdka;1突变纯合植株以及气孔世系细胞特异的CDKA;1.N146显性失活转基因植株出现了不同气孔发育异常表型，说明CDKA;1可能参与气孔多个发育阶段的调控。数据表明CDKA;1可与不同的细胞周期调控蛋白形成复合体参与不同发育阶段的细胞周期调控。我们拟利用遗传学、生物化学和细胞生物学手段，针对CDKA;1在气孔各个发育阶段的作用方式特点，寻找与其互作的细胞周期蛋白，并探讨它们与相应时期的已知气孔发育调控转录因子之间的上下游调控关系，从而勾勒出CDKA;1参与气孔发育中气孔世系细胞的分裂和分化的调控网络途径。

Stomata, which are vital shoot epidermal cell complexes of all land plants for gas exchange between plants and atmosphere, develop from protodermal cells via three formats of precursor cells after three kinds of asymmetric division and a symmetric division. The only one Arabidopsis A-type cyclin-dependent kinase CDKA;1 is a key regulator for cell division and differentiation. CDKA;1 might be involved in multiple stomatal development phases according to the findings of distinct stomatal defects from cdka;1 mutant seedlings, which is obtained by a pollen rescue technique, and from transgenic seedlings harboring dominant negative form CDKA;1.N146 in stomatal lineage cells. Our preliminary data indicated that CDKA;1 functions in cell cycle regulation at different development stages via forming a protein complex with different cell cycle regulatory proteins. Using stomatal development as the study system, we intend to use a combination of genetic, biochemical, and cell biological techniques to identify the putative CDKA;1 partner cell cycle proteins in different developmental stages, and their regulatory relationships with known stomatal transcriptional factors. Our results will lead to a hypothesis about the activity of CDKA;1 in cell division and differentiation in stomatal development and its regulatory network.