Requirement for A-type cyclin-dependent kinase and cyclins for the terminal division in the stomatal lineage of Arabidopsis.
Requirement for A-type cyclin-dependent kinase and cyclins for the terminal division in the stomatal lineage of Arabidopsis.
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拟南芥气孔谱系末期分裂对 A 型细胞周期蛋白依赖性激酶和细胞周期蛋白的需求
DOI：
10.1093/jxb/eru139
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发表时间：
2014-06
影响因子：
6.9
通讯作者：
Le J
中科院分区：
文献类型：
--
作者：
Yang K;Wang H;Xue S;Qu X;Zou J;Le J
CDKA;1 is required for the termial divison of stomatal development. The results presented here use targeted expression to fine-tune the roles of CDKs and cyclins in regulating guard cell production. The Arabidopsis stoma is a specialized epidermal valve made up of a pair of guard cells around a pore whose aperture controls gas exchange between the shoot and atmosphere. Guard cells (GCs) are produced by a symmetric division of guard mother cells (GMCs). The R2R3-MYB transcription factor FOUR LIPS (FLP) and its paralogue MYB88 restrict the division of a GMC to one. Previously, the upstream regions of several core cell cycle genes were identified as the direct targets of FLP/MYB88, including the B-type cyclin-dependent kinase CDKB1;1 and A2-type cyclin CYCA2;3. Here we show that CDKA;1 is also an immediate direct target of FLP/MYB88 through the binding to cis-regulatory elements in the CDKA;1 promoter region. CDKA;1 activity is required not only for normal GMC divisions but also for the excessive cell overproliferation in flp myb88 mutant GMCs. The impaired defects of GMC division in cdkb1;1 1;2 mutants could be partially rescued by a stage-specific expression of CDKA;1. Although targeted overexpression of CDKA;1 does not affect stomatal development, ectopic expression of the D3-type cyclin CYCD3;2 induces GC subdivision, resulting in a stoma with 3–4 GCs instead of the normal two. Co-overexpression of CDKA;1 with CYCD3;2, but not with CYCA2;3, confers a synergistic effect with respect to GC subdivision. Thus, in addition to a role in stomatal formative asymmetric divisions at early developmental stages, CDKA;1 is needed in triggering GMC symmetric divisions at the late stage of stomatal development. However, timely down-regulation of CDKA;1–CYCD3 activity is required for restriction of GC proliferation.
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影响因子：
64.5
作者：
Cruz-Ramírez A;Díaz-Triviño S;Blilou I;Grieneisen VA;Sozzani R;Zamioudis C;Miskolczi P;Nieuwland J;Benjamins R;Dhonukshe P;Caballero-Pérez J;Horvath B;Long Y;Mähönen AP;Zhang H;Xu J;Murray JA;Benfey PN;Bako L;Marée AF;Scheres B
通讯作者：
Scheres B
影响因子：
11.4
作者：
HEMERLY, A;ENGLER, JD;FERREIRA, P
通讯作者：
FERREIRA, P
影响因子：
7.2
作者：
Joubès, J;De Schutter, K;De Veylder, L
通讯作者：
De Veylder, L
影响因子：
7.4
作者：
Boudolf, Veronique;Lammens, Tim;De Veylder, Lieven
通讯作者：
De Veylder, Lieven
影响因子：
7.5
作者：
Harbour, JW;Dean, DC
通讯作者：
Dean, DC