鱼类生殖活动与能量代谢紧密相关，阐明能量代谢因子在生殖活动中的功能是解析生殖与能量代谢间调控机制的关键。本课题组前期研究发现，作为调节能量代谢的重要内分泌因子黑色素聚集激素MCH，与鲤鱼生殖活动相关。在此基础上，本课题拟开展MCH在鲤鱼生殖中的功能研究。具体内容包括：（1）克隆MCH及其受体，研究其组织分布、亚细胞定位以及与GnRH、Leptin受体细胞之间的联系，为MCH在鲤鱼生殖中的功能提供形态学依据；（2）研制转MCH基因鲤鱼，研究MCH对鲤鱼性腺发育、HPG轴生殖关键因子的作用，揭示MCH在鲤鱼生殖中的功能；（3）研究MCH对体外培养的垂体细胞LH、FSH，性腺细胞LH-R、FSH-R、雌二醇(雌)/睾酮(雄)合成和释放的影响，揭示MCH在生殖调控中的作用靶点及机理。MCH在鲤鱼生殖中的功能研究可为阐明鱼类生殖和能量代谢间的调控机制提供科学依据，为保护鱼类生殖健康奠定理论基础。

Reproduction is tightly linked to energy metabolism. It is very important to unveil the function of energy balance neuroendocrine factors in reproduction for mechanisim of pathways mediating reproduction and metabolic regulation. MCH, which is an important endocrine factor, is well established for its effects on metabolic regulation. No studies have ever reported the function of ?sh MCH with respect to reproduction. The results of our previous research showed that MCH is involved in the regulation of reproduction in common carp. In this project, the following researchs will be conducted as follows:(1) MCH and its variant receptor will be cloned, their distributions in HPG axis and connections between MCH and GnRH, and Leptin receptor will be studied. These results may provide morphological evidence for the role of MCH in the control of reproduction. (2) Transgenic carp that over-express MCH will be produced to investigate the effects of MCH on gonad development and expression of key factors involved in reproduction . These results may elucidate the role of MCH in reproduction. (3) The effects of exogenous MCH administration on the level of LH and FSH in pituitary cells, and LH-R, FSH-R and E2(female)/T(male) in germ cells will be studied in vitro. These results may reveal the targets of MCH in reproduction. These studies will provide basis for mechanism of MCH in mediating reproduction and energy homeostasis and protection of fish reproduction healthy.