Development and Characterization of Polymorphic Microsatellite Markers for Sedum sarmentosum (Crassulaceae) and Their Cross-Species Transferability.
Development and Characterization of Polymorphic Microsatellite Markers for Sedum sarmentosum (Crassulaceae) and Their Cross-Species Transferability.
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垂盆草（景天科）多态性微卫星标记的开发和表征及其跨物种可转移性
DOI：
10.3390/molecules201119669
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发表时间：
2015-11-05
期刊：
影响因子：
--
通讯作者：
Yang GZ
中科院分区：
文献类型：
--
作者：
Xu J;Hou FY;Wan DR;Wang S;Xu DM;Yang GZ
垂盆草是一种重要的中草药，具有抗炎、抗血管生成和抗伤害性作用。然而，人们对其遗传背景知之甚少。第一组14个微卫星标记被分离出来，并对S。垂盆草的SSR富集文库。获得了14个多态性微卫星标记，在48个S.垂盆草属个体等位基因数为3 ~ 15个。观察到的和预期的杂合度分别为0.0833 - 0.8750和0.2168 - 0.9063。有两个位点显著偏离Hardy-Weinberg平衡。在其他景天属植物中进行了跨种扩增。观察到跨物种扩增率很高。转移率为85.7%;在S.艾蒿这些分子标记将为研究大豆的遗传变异、群体结构和种质特性提供重要的参考。垂盆草和相关的景天属物种。
Sedum sarmentosum is an important Chinese medicinal herb that exhibits anti-inflammatory, anti-angiogenic and anti-nociceptive properties. However, little is known about its genetic background. The first set of 14 microsatellite markers were isolated and characterized for S. sarmentosum using an SSR-enriched library. Fourteen polymorphic microsatellite markers were acquired with satisfactory amplifications and a polymorphic pattern in 48 S. sarmentosum individuals. The number of alleles ranged from 3 to 15. The observed and expected heterozygosities varied from 0.0833 to 0.8750 and 0.2168 to 0.9063, respectively. Two loci showed significant departure from the Hardy-Weinberg equilibrium. Cross-species amplification was carried out in other Sedum species. High rates of cross-species amplification were observed. The transferability value ranged from 85.7% in S. lineare to 64.3% in S. ellacombianum. These markers will be valuable for studying the genetic variation, population structure and germplasm characterization of S. sarmentosum and related Sedum species.
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影响因子：
6.2
作者：
Wang, Hongxia;Walla, James A.;Dai, Wenhao
通讯作者：
Dai, Wenhao
影响因子：
5.3
作者：
Cuc, Luu M.;Mace, Emma S.;Crouch, Jonathan H.;Quang, Vu D.;Long, Tran D.;Varshney, Rajeev K.
通讯作者：
Varshney, Rajeev K.
影响因子：
7.7
作者：
Excoffier, Laurent;Lischer, Heidi E. L.
通讯作者：
Lischer, Heidi E. L.
影响因子：
2.8
作者：
Nanton, Ana;Arias-Perez, Alberto;Freire, Ruth
通讯作者：
Freire, Ruth
影响因子：
3.1
作者：
Wu, Jing;Cai, Changfu;Zhou, Hua
通讯作者：
Zhou, Hua