雄激素性脱发（AGA）患病率高，对患者生活质量影响大，深化对AGA的发病机制的认识并找到有效的治疗手段是目前临床上亟待解决的问题。国内外研究发现双氢睾酮影响毛囊毛乳头细胞（DPC）功能并促其分泌异常物质，导致毛囊上皮-间充质交流失衡，是AGA的重要成因。前期研究表明双氢睾酮处理的DPC分泌的外泌体（DHT-DPC-Exos）具有影响毛囊上皮细胞功能，为此，本课题拟通过测序、干扰、过表达和RNApulldown等实验，从细胞和动物水平分别验证DHT-DPC-Exos通过外泌体中lncRNA MEG3抑制毛囊生长发育关键基因β-catenin的表达机制；阐明DHT-DPC-Exos抑制毛囊上皮细胞功能的作用；进而解释导致毛囊上皮-间充质失衡促进脱发的机制。研究成果有望进一步丰富DPC来源的外泌体中lncRNA MEG3在AGA中的作用及机制的学术理论，为临床治疗新靶点的发现提供科学基础。

Androgenic alopecia (AGA) has a high prevalence, and has a negative impact on the life quality of patients. It is therefore an urgent problem to deepen the understanding of its pathogenesis and find effective treatment methods. Previous studies demonstrated that dihydrotestosterone (DHT) could affect the function of hair follicle (HF) dermal papilla cells (DPC) and promote the secretion of abnormal substances, resulting in the imbalance of HF epithelial-mesenchymal communication, which is an important cause of AGA. We previously found that exosomes derived from DHT-pretreated DPC (DHT-DPC-Exos) could influence the function of HF epithelial cells. Based on these findings, using sequencing, interference/over-expression and RNA pulldown techniques, we aim to clarify the mechanisms that DHT-DPC-Exos inhibits the expression of β-catenin, a key gene of HF growth and development, through lncRNA MEG3; to elucidate the role of DHT-DPC-Exos on the inhibition of HF epithelial cell function; to explain the mechanisms of hair loss caused by the imbalance of epithelial-mesenchymal interaction. Our research is expected to provide a novel understanding of the role of the exosomal cargo of DPC-Exos-lncRNA MEG3 on AGA, as well as experimental evidences for the potential new targets for AGA treatment.