细胞外基质中的蛋白聚糖分子是多种上皮间充质信号分子交流的桥梁,也是组成调控干细胞的niche的重要成分。我们前期研究发现小分子蛋白聚糖decorin具有促进毛囊休止期进入生长期的作用，本研究拟在此基础上，利用可诱导的转基因小鼠模型研究decorin过表达后引起的毛囊bulge形成及休止期向生长期转化过程中毛囊干细胞（HFSC）的动态演变；通过组织荧光检测、转录组测序及体外细胞干预实验等探索decorin通过协调TGF-β、Wnt等关键通路在激活HFSC促进休止期向生长期转化中作用；利用谱系追踪技术及毛囊重建实验在体评估decorin激活对HFSC的多潜能性及诱导能力的调控，并探索体外decorin预处理改造脱发患者功能障碍HFSC的可能性。本研究将深入揭示decorin等niche因子参与HFSC推动下毛囊周期转变的作用及机制，为利用干细胞的niche调控治疗脱发等疾病提供依据。

Proteoglycans in extracellular matrix bridge the epithelial-mesenchymal communication, and constitute niche in regulating the stem cell functions. Our prior research reveals that decorin, a small molecular proteoglycan, is able to promoting murine follicular telogen-anagen transition. Based on the previous findings, we aim to utilize the inducible transgenic mouse model to trace the dynamic change of hair follicular stem cells (HFSC) during the bulge formation and telogen-anagen transition, and we aim to explore the role of decorin in coordinating essential signaling pathways, such as TGF-β and Wnt, to activate hair follicular stem cell and induce precocious telogen-to-anagen transition, by use of tissue immunofluorescence, RNA sequencing and cellular interference techniques. Finally, we aim to utilize lineage tracing and hair follicle reconstruction techniques to evaluate the regulation of decorin activation in HFSC pluripotency and hair induce, and to explore the possibility of decorin treatment in reprogramming dysfunctional stem cells from hair loss patients. Our study will further reveal the role and mechanism of decorin and other niche moleculars in regulating HFSC and hair cycle transition, and provide the basis for the use of stem cell niche regulation in hair loss treatment.