咽喉反流性疾病（laryngopharyngeal reflux disease, LPRD）发生率高、病程长、痛苦久、危害大，但现有诊断标准和临床实际的脱节造成了LPRD诊断率和治愈率较低，导致社会医疗经济损失。本项目通过研究兔咽喉急性和慢性酸暴露后粘膜电阻抗改变及上皮屏障损害情况，比较咽喉反流患者和正常志愿者咽喉粘膜基线阻抗，证明基线阻抗值是咽喉反流疾病的一个稳定的新的诊断依据，并实现计算机算法优化和程序自动读取，并建立咽喉基线阻抗研究动物模型。

Laryngopharyngeal reflux disease (LPRD) has high incidence, long course, and many hazards. The disconnection between existing diagnostic criteria and clinical practice has resulted in a lower diagnostic rate and cure rate . What is more, it has led to social medical economic losses. This study will investigate the changes in epithelial electrical impedance and epithelial barrier damage in rabbits with acute and chronic acid exposure to compare the laryngeal mucosa baseline impedance between LPRD patients and normal volunteers. This study will focus to prove that baseline impedance is a stable new diagnostic basis for LPRD, to realize computer algorithm optimization and computer automatic analysis, and to establish a reliable animal model of laryngopharyngeal baseline impedance in LPRD.