骨折愈合这一生物学修复过程因受到诸多因素的调控。BMPs/Samd信号通路是参与骨修复、骨重建的重要枢纽，其信号途径的激活能够调节相关基因的表达，促进骨折愈合，因此BMPs/Smad信号通路的调控可能成为促进骨折愈合的新靶点。本课题基于中医“血不活则瘀不能去,瘀不去则骨不能接”和苗医“瘀毒”理论相结合，对苗药仙罗汉接骨汤在前期研究的基础上，通过免疫组化、Western blot、RT-PCR等技术研究BMPs/Samd信号通路中BMP-4、BMR1A及Smad1、5、8和Runx2 、Osterix、BSAP、OC在新西兰大白兔骨折模型中的表达，Micro-CT检测骨折模型处骨小梁的微结构，体外药效学探索苗药干预后MC3T3-E1细胞的增殖及Runx2,osterix,BSAP,OCmRNA表达，探索苗药九仙罗汉接骨汤干预骨折愈合机理，为苗药复方促进骨折愈合提供理论依据。

The biological repair process of fracture healing is regulated by many factors. Related factors in the BMPs/Samd signaling pathway are important junctions for bone repair and bone reconstruction,and activate the expression of regulated genes and achieve the goal of promoting fracture healing.Therefore, to regulate and control the BMPs/Smad signaling pathway may be a new target for the promotion of fracture healing. This research is based on the theory of TCM"blood does not live, and the blood stasis can not go, the blood stasis can not be removed"and combine the "stasis toxin" theory of miao medicine. In Guizhou Miao medicine as the main composition of Jiuxianluohanjiegu Decoction, On the basis of previous research,it research the proliferation and mRNA expression of Runx2, Osterix, BSAP and OC to the MC3T3-E1 cells after the treatment of miao medicine by means of the Efficiency Evaluation of extracorporal pharmacodynamics. through immunohistochemical technique, Western blot, RT-PCR methods research the expression of BMP-4, BMR1A, Smad1,5,8,Runx2, Osterix, BSAP and OC from the New Zealand white rabbit fracture model, and detect the bone trabecular microstructure by the Micro-CT, further, to explore miao medicine Jiuxianluohanjiegu Decoction's Mechanism to promote fracture healing, it can provide a new basis for the Miao herbal medicine prescription promot fracture healing.