年龄相关性白内障(age-related cataract，ARC)是由多种因素引起的晶状体部分或全部浑浊，引起视力降低的全球性致盲眼病。目前主要是以手术治疗为主，但一方面，我国每年ARC患者数量与眼科医疗资源总量不足、分布不均的困局不匹配，另一方面，ARC手术和耗材费用负担沉重。更重要的是，手术存在不可避免的并发症，而且还存在很多非手术适应症的ARC患者。前期研究发现：补青颗粒可干预大鼠晶状体氧化应激损伤，并且可调控早中期糖尿病小鼠细胞自噬水平保护晶状体。结合国内外研究，我们发现CRYAA基因缺陷是ARC发生的重要原因，而细胞自噬引起晶状体内环境改变是ARC进展的诱因之一。故本研究拟利用CRISPR/Cas9技术，制备出CRYAA基因修饰的白内障家兔模型，从动物在体-细胞离体-分子水平进行研究，进一步证实“补青颗粒可通过自噬相关通路改善ARC”的假说。

Age-related cataract (age - related cataract, ARC) is caused by many factors in some or all of the turbidity of the lens, which is the global-blinding eye disease reducing vision. At present, surgery is the main treatment method, but on one hand, the annual number of the patients insufficient ARC of China does not match with ophthalmic medical resources, uneven distribution of woes, on the other hand,the ARC surgery and consumable costs heavy. And more importantly, complication is inevitable, but also has a lot of patients with non-surgical indications of the.ARC. Preliminary study found: fill green particles can intervene in the rat lens oxidative stress damage, and can regulate early metaphase cell autophagy level protection lens diabetes mice. Combined with the domestic and foreign research, we found that CRYAA genetic defects are one of the important reasons for the ARC, and cause the lens cell autophagy internal environment change is one of the cause of.ARC progress. Therefore, the research intends to utilize CRISPR/Cas9 technology, prepared CRYAA cataract rabbit model of genetic modification from animal in vivo cells in vitro molecular level, further confirmed the hypothesis that "Bu Qing Particles improve the ARC by autophagy related pathways".