基于宁夏特色中药防治西北地区肺系时疫创新药物的应用基础研究

历次瘟疫表明，肺系时疫成为威胁人类健康的重大疾病,而中医药在防治疫病中呈现出独特优势,前期研究已证实由宁夏特色中药组成的麻杏苦甘汤对西北肺系寒燥时疫疗效确切。但其有效组分、配伍机制及安全性与毒性缺乏全面系统研究。因此拟开展:1.基于高通量、高内涵筛选系统,以寒燥疫毒袭肺病症结合模型及急性肺损伤等动物模型评价方法和技术,开展功效导向下的特色中药活性部位及活性成分研究;2.基于方剂配伍理论与组分配伍思想,开展特色中药及活性组分配伍效应研究,优化量-效关系,阐明其配伍机制;3.基于多组学技术,阐明功效物质的体内代谢转化特征、调控生物网络关系、作用靶标及信号通路调节等化学生物学机制;4.基于药物安全性评价原则,开展活性组分毒性与机制研究,组分配伍安全性评价等成药性研究。以期在阐明麻杏苦甘汤功效物质、作用机制基础上,通过精当配伍,筛选用于防治我国西北肺系寒燥时疫的中药组分配伍候选药物。

Infectious diseases show that pulmonary epidemic disease has become a major disease threatening human health, and Traditional Chinese Medicine has a unique advantage in fighting against the epidemic disease. Previous studies have proved that Ma Xing Ku Gan tang, which is composed of Traditional Chinese Medicine with Ningxia characteristics, has a definite effect on pulmonary epidemic disease in northwest China.This project is based on our country northwest pulmonary pestilence cold-drying characteristics, such as Ephedrae Herba,Stellariae Radix, Sophorae Alopecuroidis Semen， characteristics of Traditional Chinese Medicine as research object, to carry out: 1. Based on the high flux, high content screening system and combining the cold-dry plague poison attack pulmonary disease and acute lung injury animal models combined evaluation methods and techniques, to carry out the functions under the guide of characteristic Chinese medicine active site and active ingredient research;2. Based on the compatibility theory of prescriptions and the idea of compatibility of components, carry out research on the compatibility effect of characteristic Traditional Chinese Medicine and active components, optimize the relationship between quantity and effect, and clarify the compatibility mechanism;3. Based on the multi-omics technique, elucidate the chemical biological mechanisms such as in vivo metabolism and transformation characteristics of functional substances, regulation of biological network relationships, action targets and regulation of signal pathways;4. Based on the principle of drug safety evaluation, studies on the toxicity and mechanism of active components, as well as the evaluation on the safety of compatibility of components and other studies on medicinal properties were carried out.Based on the elucidations of the effective substances and mechanism of Ningxia characteristic Traditional Chinese Medicine, we hope to create an innovative drug for the compatibility of Traditional Chinese Medicine components for the prevention and treatment of pulmonary diseases induced by dry-cold climate in northwest China.