肿瘤微环境中诱导分化的肿瘤相关Treg已成为肿瘤免疫治疗的主要障碍，我们前期的研究提示，卵巢癌微环境中存在卵巢癌特异性Treg，有助于卵巢癌免疫抑制微环境的形成，抑制抗肿瘤免疫应答。最近研究提示通过TLR信号途径调控肿瘤Treg功能可为肿瘤免疫治疗提供新思路。在本研究中，我们从细胞克隆水平对卵巢癌特异性Treg的表型、细胞因子分泌特点、功能及其作用机制进行深入研究；在此基础上，筛选卵巢癌特异性Treg高表达的TLR，从细胞水平和动物水平上探讨其在卵巢癌特异性Treg功能调控中的作用及其信号途径。研究结果将有助于阐明卵巢癌免疫逃逸及免疫抑制机制，以及TLR在卵巢癌特异性Treg功能调控中的作用，这不仅为卵巢癌特异性Treg功能调控所介导的卵巢癌免疫治疗奠定理论基础，而且为治疗性疫苗的研发提供新策略，也为其他肿瘤的免疫逃逸机制及免疫治疗的研究提供新视角。

Tumor associated Treg induced in tumor microenvironment become the major obstacle in tumor immunotherapy. Our previous study revealed that Treg specific to ovarian cancer maintain immunosuppressive milieu and suppress anti-cancer responses. Recent study suggested that TLR signaling may play the important role in the functional control of tumor associated Treg, which offer new opportunities to improve the outcome of cancer immunotherapy. In this study, we will explore the phenotypes, cytokine profiles, suppressive effects and suppressive mechanisms of Treg cell clones specific to ovarian cancer. Moreover, we will screen and choose the TLR with high levels to explore the control function and the signaling pathway of which in regulating Treg specific to ovarian cancer. Finding of this study not only provide the basis for cancer immunotherapy by Treg, but also provide new strategy for therapeutic vaccine, and provide point of view for studying on mechanism and immunotherapy of other tumors.