花生是我国重要的油料经济作物，降低硬脂酸、提高油酸含量是目前花生遗传改良的重要目标之一。FAB2编码硬脂酰-ACP脱饱和酶，位于油酸合成通路的上游，调控硬脂酸向油酸转化。前期我们发现高油酸通过ROS信号途径反馈调节FAB2的表达，随后利用酵母单杂交我们鉴定到FAB2的转录因子AHL23。AHL23编码蛋白含有保守的AT-hook以及DUF296结构域，主要被定位在细胞核。AHL23的表达模式受油酸调控，在高油酸花生种子发育过程中AHL23表达量显著低于正常品种。本研究拟利用遗传学、分子生物学、以及基因编辑等方法全面系统地解析AHL23的分子功能，重点阐明AHL23响应上游ROS信号调节下游FAB2表达，进而影响花生硬脂酸-油酸含量变化的分子机制。通过项目的开展，我们希望在理论上拓展对AHL23的功能认知，在应用上为进一步改良高油酸花生的品质提供理论指导。

Peanut (Arachis hypogaea L.) is a pivotal commercial oilseed crop in China, which is one of the most important goals to decrease stearic acid and increase the oleic acid contents of peanut seed in genetic improvement. Stearoyl-ACP desaturase, encoded by FAB2, which located on the upstream of oleic acid biosynthesis pathway, modulates the conversion of stearic acid into oleic acid. Previously, we found that the high-oleic feedback regulated the expression level of FAB2 via ROS signaling pathway, and then we identified an AHL23 as the transcription factor of FAB2 by Yeast one-hybrid. AHL23 encoding protein contained conversed AT-hook and DUF296 domain, was localized into nucleus. The expression pattern of AHL23 was modulated by oleic acid, which expression level was significantly decreased in the developing high-oleic peanut seeds as comparsion with normal peanut. In this study, we intend to utilize the methods of genetics, molecular biology and genome.editing to comprehensively and systematically elucidate the molecular function of AHL23, which emphatically illustrate the molecular mechanism of AHL23 responds to upstream ROS signaling and regulates downstream FAB2 expression, thereby leading to the change of stearic acid and oleic acid contents in peanut. Through this project, we hope to expand the functional cognition of AHL23 and provide a theoretical direction for futher improve th quality of high-oleic peanut varieties.