长期以来，对糖蛋白的研究受限于糖链均一蛋白质的获取，发展高效合成糖基化蛋白的技术将对生命，医疗健康有重要的意义。糖蛋白的合成需要兼顾糖类合成，多肽合成和基因重组技术，国内外仅有极少数实验室有能力合成结构完整的复杂糖蛋白。鉴于糖蛋白在生命体系内的重要性，利用生物与化学合成相结合的方式，发展新颖的技术获取糖基化蛋白，将为探索生命现象提供有力的工具。本研究项目具有重要的科学意义和原创性，对该领域以及相关的合成生物学领域研究起到促进作用。本项目以白细胞介素15（IL-15）为目标分子，综合基因表达方法与有机合成等手段，精确、高效合成具有均一化糖链结构的糖蛋白，阐明糖链结构对该重要蛋白功能活性的影响。同时，合成过程中发展的方法学将对精确合成糖蛋白这个领域起到推动作用。

Over fifty percent of human proteins are glycoproteins. But for many years, research progress of glycoproteins is impeded by the heterogeneity in their oligosaccharide. Glycosylation, one of the posttranslational modification, is based on a complex biosynthetic machinery. Development of efficient methods for the synthesis of homogeneous glycoprotein is very important for life science, protein science and glycobiology. Benefit from the technology of carbohydrate, peptide synthesis and protein expression, now scientists could synthesize homogeneous glycoproteins, though only few labs in the world have the abilities to integrate these technologies to obtain homogeneous glycoproteins. Semi-synthesis of glycoprotein with precise structure molecule by protein expression and chemical synthesis of glycopeptides, will afford a powerful tool for the discovery of life science. .Interleukin-15 plays many key roles within both innate and adaptive immunity, which is currently evaluated in several clinical trials. The pharmacokinetic properties of IL-15 are associated with its glycosylation, which remains largely unclear. We will use expressed protein ligation and chemical synthesis of N-linked glycan, to synthesize homogeneous IL-15, address its structural-functional relationships, that may allow us to improve therapeutic properties of IL-15.