线虫是生态系统重要组成部分和动植物病原物，捕食线虫真菌通过菌丝特化成的捕食器官捕捉线虫，在自然条件下对线虫动态起重要调控作用。收缩环捕捉线虫时，环细胞在0.1-1秒内膨胀三倍以上，前期代谢抑制研究表明细胞骨架、钙离子通道及磷酸化酶与环细胞的感触反应及收缩有关。本项申请以Drechslerella stenobrocha为研究对象，通过对actin蛋白进行荧光标记结合显微观察，明确细胞骨架微丝蛋白在感触反应中的作用；通过基因敲除技术，明确钙离子通道、G蛋白偶联受体及酸磷酸化酶在收缩环感触反应及收缩过程中的作用；通过孢子捕食器官转录组分析，并结合代谢抑制剂验证，最终解析收缩环感触反应及细胞膨胀的分子机制。研究结果不仅对研究真菌感触反应和细胞形态变化具有重要意义，而且丰富了真菌捕食及生物感触反应的知识。

Nematodes are essential component of ecosystem and important pathogens of animals and plants. Nematode-trapping fungi regulate nematode dynamics in nature by capturing nematodes with special trapping devices developed from hyphae. The constricting ring as sophistical trapping device composed by 3 cells which rapidly triple inflated their volume within 0.1 to 1s stimulated by the nematodes. Our initial metabolic inhibitor experiment indicated that the ring thigmonasty is related to cytoskeleton, calcium ion channels and phosphorylases. The aims of current proposal are 1) to investigate the role of cytoskeleton microfilament proteins in thigmonastic response by actin fluorescent labeling technique; 2) to identify the functions of related genes involving in calcium ion channel, G-protein-coupled receptors and phosphorylases by gene knock out technique; 3) to reveal ring thigmonastic mechanism by transcriptome analysis of conidia constricting ring and metabolic inhibitor bioassay. The results are not only providing new insightthigmonastic response and morphological changes of fungal cells, but also enrich knowledge of fungal carnivorism and biological thigmonastic response.