真菌性角膜炎是严重致盲性眼病。固有免疫是角膜抵抗真菌感染的第一道防线，模式识别受体是固有免疫的中心。本研究拟从动物模型的阻断实验、过表达实验以及FK患者及动物模型的表观遗传学等方面分析跨膜型模式识别受体LOX-1在人角膜抗真菌固有免疫的作用机制，通过Confocal显微镜、Western blot、ELISA、Real-time PCR等分子生物学手段等方法，以LOX-1、下游细胞因子以及粘附功能为评价指标，分析LOX-1与NFκB、细胞因子、趋化因子之间的关系以及与TLR4在固有免疫中的相互作用，分析LOX-1在角膜抗真菌固有免疫中的重要性，观察LOX-1对真菌性角膜炎病变过程的影响，论证LOX-1介导的固有免疫在真菌性角膜炎中的作用及机制，从而进一步深入研究真菌性角膜炎发病机制，为真菌性角膜炎的治疗提供新的思路。

Fungal keratitis (FK) is a severe blinding disease. Innate immunity is the first line to defense against fungal infection of cornea, and pattern recognition receptors are the center of innate immune. This topic intend to analysis the role of lectin-like oxidized low-density lipoprotein receptor (LOX-1) and explore the influence of epigenetics in antifungal immunity of cornea by application of animal models with fungal keratitis. The signaling pathway is analyzed by the usages of antibody blocking, double-stranded RNA inference, overexpression, inhibitor, and by the methods of confocal microscopy, Western blot, ELISA, Real-time PCR, et al. Take several key targets of molecule signaling pathway, cytokines downstream and adhesion function as detection indicators and explore the relationships among LOX-1, NFκB, cytokines and chemokines and interaction of LOX-1 with TLR4 in innate immune. Determine the special significance of LOX-1 in the complex network. Observe the influence of LOX-1 in the course of fungal keratitis. Thus comprehensively study LOX-1-mediated innate immune in mechanism of fungal keratitis, so as to provide new idea for the treatment of fungal keratitis.