HBSP(helix B surface peptide)是我们自主合成并已申请专利的肾脏保护性多肽，前期研究已证实其在肾脏缺血再灌注损伤中具有显著的抗凋亡和抗炎症的保护作用，但其对胞内信号通路的影响机制不明。目前我们已建立猪肾冷保存及自体移植模型，猪多样化的基因背景及与人接近的肾脏结构均使其作为研究HBSP肾脏保护机制模型具有独特优势。因此，本课题拟在猪自体肾移植模型中验证HBSP对移植肾的保护效应，并进一步应用蛋白组学技术探索HBSP在移植前后不同时间节点所引起的肾脏内蛋白表达谱变化，通过对差异蛋白的分析比对筛选出HBSP所作用的胞内信号通路，绘制HBSP动态胞内信号转导机制网络图。在此基础上，我们将在体内运用基因沉默技术siRNA对信号通路上关键接头蛋白的表达进行干预，验证信号通路，最终深入阐明HBSP对肾脏的保护作用机制，为HBSP的临床转化提供理论依据。

Helix B surface peptide (HBSP) is a novel renoprotective peptide,synthesized by our group and its patent received. Our preliminary studies have confirmed significant anti-apoptotic and anti-inflammatory effects of HBSP in renal ischemia reperfusion injury, but its intracellular signaling pathway still needs further research. We have established the kidney cold preservation and autotransplantation model in porcine. Porcine, as a research platform, displays unique advantages of diverse genetic background and its structural similarity to human kidney. Therefore, this project intends to evaluate the renoprotective effect of HBSP in the porcine autotransplantation model and to explore its mechanism. We will analyze HBSP-induced kidney protein expression changes on different timepoints with iTRAQ (isobaric tags for relative and absolute quantitation) labeling and quantitative proteomic technology, and screen out potential intracellular signaling pathways of HBSP by identifying differentially expressed proteins. We will further use siRNA to interfere with the expression of key molecules in the newly discovered signaling pathways in vivo for verification. This project aims to clearify the renoprotective mechanism of HBSP, providing theoretical support for its clinical translation in kidney transplantation.