我们前期研究发现鼻咽癌中EBV-LMP1参与了癌细胞Warburg效应及肿瘤相关免疫细胞分化及功能的调控；且肿瘤Warburg效应与免疫耐受的发生有密切关系，但鼻咽癌细胞表达LMP1与Warburg效应及肿瘤免疫耐受三者间的关联性及分子机制尚不清楚。我们提出鼻咽癌通过表达LMP1促进癌细胞发生Warburg效应，使癌细胞发生细胞因子分泌等生物学行为改变，诱导肿瘤微环境免疫细胞（MDSC，巨噬细胞，Treg，CTL等）的分化与功能失衡，导致肿瘤免疫逃逸的科学假设。我们首先通过细胞共培养、RNA表达谱芯片、靶基因过表达及敲低等方法分析LMP1表达对鼻咽癌细胞糖酵解及肿瘤相关免疫细胞分化与功能的作用和调控机制；其次通过靶基因干预和相关体内实验探讨癌细胞表达LMP1、糖代谢失调与免疫耐受在鼻咽癌临床进程和治疗中的作用，预期发现癌细胞表达LMP1调控糖代谢失调参与鼻咽癌免疫耐受的分子机制和干预靶点。

Our previous study identified that the latent membrane protein (LMP1) is an important oncogene and target antigen in Epstein-Barr virus associated cancers including nasopharyngeal carcinoma (NPC), which regulated the Warburg effect of tumor cells and the differentiation and function of cancer-associated immune cells. In addition, it is revealed that the Warburg effect of tumor cells is correlated with cancer immune tolerance, however, the association and its regulated mechanism between the expression of LMP1 and Warburg effect as well as cancer immune tolerance in NPC is still unclear. Based on the previous study, we proposed the hypothesis that the expression of LMP1 in NPC cells induced the imbalance of differentiation and function of cancer-associated immune cells, including MDSC, macrophage, Treg and CTL cells, through up-regulation of Warburg effect of tumor cells, which leading to the abnormal biological function of tumor cells including cytokine secretion. We determined the mechanisms of LMP1 promoting the aerobic glycolysis of NPC cells, and inducing the differentiation and functional impair of cancer-associated immune cell subsets by RNA expression microarray, overexpression or knock-down of target genes and cell coculture. We investigated the association between the LMP1, Warburg effect and tumor immune escape as well as their role in the disease progression of NPC by RNA interference, clinical relevance, and animal model. We aimed to find out that the molecular mechanism of the expression of EBV-LMP1 in NPC cells regulated the immune tolerance of NPC by regulating the reprogramming of glucose metabolism of tumor cells, and some target genes which maybe provide a potent value in immunotherapy of NPC.