畜禽生产中由大豆抗原引发的过敏现象普遍存在，且不同种属动物对大豆敏感性差异悬殊，但导致这些差异的相关机制尚不清楚，了解这些机理对于合理利用大豆蛋白饲料有重大经济价值和理论意义。本项目选取不同种属动物(仔猪、犊牛、雏鹅、鼠）饲喂去皮豆粕，通过检测腹泻率、皮肤试敏、血清总IgE和特异性IgG含量，来比较不同种属动物对大豆抗原致敏反应的差异；采用生物信息学法、免疫亲和质谱技术、ELISA等方法预测和鉴定大豆抗原蛋白β-Conglycinin的抗原表位；采用ELISA法，蛋白印迹法、免疫组化法检测小肠上皮与β-Conglycinin抗原表位结合受体的表达与分布情况。本项目将揭示不同种属动物对大豆抗原蛋白致敏差异的相关机制，这为日后抗原纯化、定量检测、寻求有效过敏阻断剂提供理论基础，并为深入揭示大豆过敏机理服务。另外，项目对于丰富比较动物营养学理论、优化动物饲料利用、保障饲料及食品安全均有重要意义。

Soybean-induced allergic reaction is of common occurrence in animal production, and there occurs great different sensitivity to soybean between different animal species. However, its allergic mechanism is not clear to us. So finding out the relevant mechanism brings us economic significance and theoretical value for soybean protein feed utilization. In this study, different animal species (piglets, calves, chicken, mice) were selected to feed the dehulled soybean meal in order to compare the different sensitivity to soybean allergen by recording the diarrhea rate, sensitive skin test, sera IgG and specific IgE content. The β-Conglycinin epitope would be predicted and identified using the methods of bioinformatics, immunoaffinity mass spectrometry, and ELISA. The expression and distribution of epitope-binding receptor would be compared in the intestinal epithelium of different animal species by ELISA, western Blotting, and immunohistochemistry. These results would reveal the difference of β-Conglycinin–induced allergic reaction between species, which could provide the basis of antigen purification, quantity detection and seeking effective blocker, and further explore the allergic mechanism. Furthermore, it is significant to enrich the theory of comparative animal nutrition, improve feed utilization and food/feed safety.