天女木兰MsARF5基因在后熟种子胚胎发育中的功能及其作用机制

天女木兰是我国重点保护的集观赏、芳香和药用价值于一身的珍稀濒危野生木本植物资源，具有广阔的开发应用前景。该树种以播种繁殖为主，种子具深休眠特性，种胚发育不完全致使种子萌发、出苗困难，极大限制了开发应用进程。至今，对天女木兰种子后熟期间种胚发育调控机制仍不清楚。申请者在天女木兰种子转录组测序数据基础上，深度挖掘种胚发育相关的差异基因，通过NCBI数据库比对并参考项目组前期研究结果，发现生长素响应因子ARF5随种胚发育显著上调表达。因此，本项目拟从天女木兰MsARF5基因入手，通过基因克隆、基因功能、表达调控机制以及MsARF5调控的目标靶基因等方面开展研究，旨在明确MsARF5基因的生物学功能、探明其在后熟种子胚胎发育中的作用，揭示MsARF5基因调控种子胚胎发育的分子机理，为具有后熟特性种子的种胚发育调控提供科学的借鉴和参考。

Magnolia sieboldii is a kind of rare and endangered wild plants resources tree species which focus on the protection of ornamental, aromatic and medicinal value in oneself, It has broad development prospects. This tree is given priority to with seed propagation, incomplete development of embryos lead to seed germination and seedling emergence difficult, greatly limiting the development and application processes. So far, during after-ripening process embryo development mechanism remains unclear. Based on seed transcriptome sequencing data, depth of excavation embryo development differentially expressed genes, comparing the NCBI database and refer to the results of previous research applicants found that Auxin response factor (ARF) significantly upregulated expression with embryo development. Therefore, this project intends to start from MsARF5 gene. Through cloning, gene function, expression mechanism and MsARF5 control goals target genes, the applicant sought to determine the biological function of genes MsARF5, prove its role in the after-ripening seed embryo development and reveal the molecular mechanism of MsARF5 regulation embryo development. This research provides a scientific reference of after-ripening embryo development.