鉴于祖国医学敷脐疗法的独特优势，本项目选择神阙穴敷贴防治术后肠动力障碍（POI）临床疗效确切的参黄方为代表方药，以离体小型猪为评价模型、非穴位皮肤为对照，采用激光共聚焦显微结合电子扫描显微镜技术，以参黄方中大黄素、丹参素等有效成分的经皮渗透速率及皮肤各层药物分布为指标，比较神阙穴和非穴位皮肤的药物渗透特性和组织结构差异性；继而复制POI家兔模型，非穴位给药为对照，构建多组分网络靶点PK-PD模型，多维、整体表征神阙穴贴敷参黄膏对POI的效应机制。本项目基于中药复方整体性特征，系统研究神阙穴与非穴位给药之间皮肤结构、经皮渗透特性和PK-PD差异性，有望阐明参黄方神阙穴给药治疗POI的作用机制，揭示中医敷脐疗法的科学内涵，还可为穴位给药的临床推广提供理论与实验依据。

In view of the unique advantages of umbilical compress therapy, a Chinese herbal medicine Shenhuang from Shenque acupoint sticking was chosen as the representative in this project,which had definite clinical effect in treating POI. Minipig belly button skin in vitro was used as the evaluation model, non-acupoints skin was used as contrast. Laser scanning confocal microscopy combines scanning electron microscopic techniques were used in this project. The transdermal osmotic rate and the drug distribution in each layer of skin of emodin and tanshinol were used as indicators to compare the differences of drug penetration and organization structure between Shenque acupoint skin and non-acupoints skin.After Shenhuang plaster applied to the minipig in vivo, microdialysis combines LC-MS technique were used to study the difference between Shenque acupoint skin and non-acupoints skin,and to study the correlation of skin electrical resistance.Rabbit models of POI were copied in this project,and non-acupoints skin dose was used as contrast to build multicomponent network targets PK-PD model and integrally represent the effector mechanism of Shenhuang paste from Shenque acupoint sticking in treating POI.This project based on the ntegrality of Traditional Chinese medicine compound,systematically and thoroughly studied the differences in skin structure,percutaneous permeability,skin pharmacokinetic and PK-PD between Shenque acupoint skin and non-acupoints skin dose,expected to illustrate the effector mechanism of Shenhuang doses in Shenque acupoint,to uncover the scientific connotation of the reason of Shenque acupoint in treating diseases,and also to provide experimental basis for TCM acupoint drug administration's clinical promotion.