长链非编码RNA(LncRNA)与宫颈癌转移密切相关。研究表明竞争性内源RNA(ceRNA)是一种全新基因表达调控模式，但LncRNA作为ceRNA在宫颈癌研究甚少。申请人在上一项国自然中筛查出LncRNA799在宫颈癌表达上调，功能研究证实其可通过上调TBL1XR1促进宫颈癌转移。近日，我们通过生信分析发现LncRNA799和TBL1XR1序列都存在miR-454-3p应答元件，预实验发现三者可与Ago2蛋白形成复合物。据此，我们推测LncRNA799可能作为ceRNA介导miR-454-3p调控TBL1XR1促进宫颈癌转移。本项目拟首先通过Ago2-RIP、荧光素酶报告及体内外分子生物学等实验技术，从分子结构、细胞功能及生物效应上证实LncRNA799具有ceRNA特性；然后筛查验证TBL1XR1下游信号通路，阐明其促进宫颈癌转移的分子机制。本项目对丰富宫颈癌转移机制具有重要理论意义。

Long chain non-coding RNAs (LncRNAs) are closely associated with cervical cancer metastasis. Some lncRNAs act as competitive endogenous RNAs (ceRNAs) to regulate gene expression. However, few studies have investigated lncRNAs as ceRNAs in cervical cancer. A national natural science foundation project identified lncRNA799 as being expressed in cervical cancer. A functional study confirmed that lncRNA799 increases the expression of TBL1XR1 (encoding transducin beta like 1 X-linked receptor 1) to promote metastasis in cervical cancer. Recently, using bioinformatics, we found that the lncRNA799 and TBL1XR1 sequences both have an miR-454-3p response element. Preliminary experiments found that these three RNAs form protein complexes with Ago2. Therefore, we speculated that lncRNA799 may act as a ceRNA to mediate miR-454-3p’s regulation of TBL1XR1, thus promoting cervical cancer metastasis. This study will use Ago2-RNA immunoprecipitation, luciferase reporters, and other molecular biology techniques, in vivo and in vitro, to confirm the ceRNA characteristics of lncRNA799 in terms of its molecular structure and cellular biological functions. We will then screen and verify the TBL1XR1 downstream signaling pathways to clarify the molecular mechanism whereby TBL1XR1 promotes cervical cancer metastasis. Understanding the mechanism cervical cancer metastasis has important theoretical and clinical significance.