当前林业苗圃生产中连作障碍导致的问题主要包括：土传病害发生严重、土壤理化环境破坏、苗木产量和品质下降，亟待开发环境和谐性微生物修复剂来克服连作障碍、控制病害发生、提高苗木质量。深色有隔内生真菌（DSE）是植物重要的根际微生物类群，在提高苗木抗逆性、修复土壤微生态环境方面发挥重要作用。本项目在前期研究的基础上，通过诱导分离和筛选针叶苗木立枯病生防DSE菌株，利用semi-pre HPLC技术、共聚焦激光扫描显微技术，从竞争、抗生以及水培苗根际互作的组织学探讨DSE对立枯丝核菌的抑制机理，利用生物化学分析技术、转录组测序技术研究DSE-樟子松共生体对病原菌生物胁迫的响应模式，在DSE与病原菌互作、DSE-樟子松共生体与病原菌互作两个层面明确DSE提高樟子松苗木抗病性的生理和分子机制，为进一步研制对植物具有促生抗逆作用的微生物菌剂提供理论基础。

The main problems in forestry nursery caused by continuous cropping obstacle include: disease occurred seriously, Soil physical and chemical environmental damage, decline of seedling yield and quality. It is necessary to research environmental harmony and bioremediation agent to control soil-borne disease and overcome continuous cropping obstacle. Dark septate endophytes(DSE) is one of most important rhizosphere microorganism groups of conifers, and have some positive contributions to host include control soil-borne disease and enchance host enviroment tolerance. This project plan to research the interaction of DSE and host plant, study the effect between DSE and Rhizoctonia solani from the aspect of competitive effect, antibiotic action and rhizosphere interaction. biochemical analytic and transcriptome sequencing technology will be employed to research the physiological and molecular response of DSE-Pinus symbiont under the stress of Rhizoctonia solani. In the end, clear and definite the physiological and molecular mechanism of Pinus sylvestris var. Mongolica disease resistance enhanced by DSE. In order to provide theoretical basis to develop the plant promote and stress resistance microbial agent.