微创外科是外科治疗的趋势和发展方向，研制一种可注射的组织工程骨的构建方法用于骨不连和骨缺损的治疗具有广阔的应用前景。但是，分散的种子细胞和外源性的细胞载体的应用严重限制了可注射组织工程骨的发展。为了克服上述缺点，本项目针对可注射组织工程骨再生的瓶颈问题，拟选择冻干富血小板纤维蛋白干粉和成骨性脂肪干细胞聚集体分别作为骨组织工程中的支架材料、生长因子和种子细胞。主要研究以下问题：1. 成骨性ADSCs聚集体的构建；2. 冻干PRF体外对ADSCs增殖和分化能力的影响；3. 成骨性ADSCs聚集体和冻干PRF复合体注射移植到裸鼠皮下后的成骨效果；4. 该复合体对兔下颌骨骨不连模型的修复效果；5. 该复合体对兔下颌骨牵张成骨模型有无骨再生的促进作用。本项研究首次提出利用冻干PRF干粉和成骨性ADSCs聚集体的生物学特性，探讨可注射组织工程骨的构建方式，为基于可注射组织工程骨的微创外科提供理论依据。

With the increasing popularity of minimally invasive surgery, to fabricate an injectable bone would be very promising and significant for the reconstruction of bone nonunions and defects. However, the application of dispersed seed cells and exogenous cell carriers severely limits the development of injectable tissue engineered bone. To overcome these limitations, lyophilized Platelet-rich fibrin (PRF) and osteogenic ADSCs aggregates were selected as the scaffold, growth factors and seed cells in this study, respectively. We plan to investigate these issues as follows: 1. the fabrication of osteogenic ADSCs aggregates; 2. the effect of lyophilized PRF on the proliferation and differentiation capacity of ADSCs in vitro; 3. the osteogenic potential of ADSCs aggregates - lyophilized PRF complex after they were injectable transplanted subcutaneously in nude mice; 4. the repair effect of this complex on the mandibular fracture gap of delayed healing model in New Zealand Rabbit; 5. the repair effect of this complex on the mandibular distraction osteogenesis model in New Zealand Rabbit. This study explored the construction of injectable tissue engineered bone based on the biological characteristics of lyophilized PRF and osteogenic ADSCs aggregates, and provided a few theoretical basis for minimally invasive surgery based on injectable tissue engineered bone..  We believe that this program will explore an efficacy and safety regenerative approach for bone tissue.