长非编码RNA(lncRNA)是长度>200核苷酸、不编码蛋白的转录本，在植物中发现大量存在。植物进化形成5大类群（藻类、苔藓、蕨类、裸子、和被子植物）的过程中，在从水生到陆生、从叶状体到有维管的根茎叶分化、从配子到开花种子形成，伴随有大量新lncRNA出现和演化。本研究聚焦植物lncRNA系统进化和功能的关键问题，通过构建覆盖全部5个植物类群/17个物种的模型体系，开展国际上迄今最完整体系的、植物lncRNA演化的比较基因组学大数据研究。内容：1）实验获取5类17种植物组织的方向特异性转录组数据；结合基因组筛选lncRNA并构建植物lncRNA数据库。2）开展植物lncRNA聚类和进化分析，揭示类群特异性lncRNA及其与5大植物类群性状发生的关系。3）构建lncRNA和编码RNA的互作调控网络；解析重要功能lncRNA时空分布和形成，并利用CRISPR技术进行分子遗传功能分析。

Long non-coding RNAs (lncRNAs) are defined as non-protein coding transcripts longer than 200 nucleotides, which are found to have large number in green plants. Plants formed 5 major groups through evolution: alga, moss, fern, gymnosperm, and angiosperm, which migrated from water to land, formed root, stem and leaves with vascular structure, and developed from gametophyte to flower seed. During the process, there are large number of lncRNA appeared and evolved. This study is designed to focus on the important question on system evolution of plant lncRNA, taking advantage of plant models for 5 major groups with 17 species. This represents so far in the world, the most comprehensive big omic-data study on evolution and function plant lncRNA. .Major contents include: i) We will carry out directional transcriptome sequencing on 17 representative species; We will systematically screen and uncover plant lncRNAs, and establish a plant lncRNA database for them. ii) We will perform analysis on the origin, evolution and clusters of plant lncRNAs, and on the association between the occurrence of five major plant groups and group-specific lncRNA. iii) We will construct interaction and regulation networks for lncRNAs and coding RNAs, and resolve the spatial and temporal distribution and metastasis of plant functionally important lncRNA during plant growth and development. Taking advantage of CRISPR-mediated molecular technique on genetic manipulation, we will perform analysis with model plants on the functions of important plant lncRNAs.