更年期综合征是女性常见病症，临床上多采用激素替代疗法(HRT)，但鉴于HRT的风险性，寻求更为安全的有效药物已成为该领域的研究热点。相比活性较弱的单一类别的植物雌激素，多成分多靶点作用的中药复方显然更具优势。中医以温补肾阳为更年期综合征的主要治则，青娥丸为补肾良方，由杜仲、补骨脂、核桃仁和大蒜组成，除含有丰富的黄酮类和香豆素类等植物雌激素外，还含有木脂素类、环烯醚萜类等成分可多途径多靶点缓解更年期症状。由于更年期综合征的症状分布广泛，单一指标很难全面反映疗效，因此我们引入与更年期综合征密切相关的神经-内分泌-免疫(NEI)网络，能够反映机体的整体状况，是评价更年期综合征疗效的理想指标。本研究拟通过青娥丸对去势大鼠进行干预，基于NEI网络全面评价青娥丸治疗更年期综合征的疗效，并通过与尼尔雌醇（雌激素）和大豆异黄酮（单一类别植物雌激素）的药效进行对比，深入剖析中药复方多成分多靶点的治疗优势。

Menopausal syndrome is a common disease of women, and is usually treated with hormone replacement therapy (HRT) in the clinical. However, in view of the risk of HRT, Seeking more safe and effective drugs has become a focus in this research field. Due to the single class of phytoestrogen often has weak active, Traditional Chinese prescriptions with multi-component and multi-target have attracted much more research interest.The principle for the treatment of menopausal syndrome in TCM is warming kidney. Qing'E pill has the function of invigorating the kidney, is a compound prescription comprised of four ingredients, Eucommniae Cortex, Psoraleae Fructus, Juglandis Semen and Garlic Rhizoma. Qing'E pill is rich in phytoestrogens such as flavonoids and coumarin, and also contains other compounds such as lignans and iridoid, which can relieve menopausal symptoms from the multi-channel and multi-target.Because the symptoms of menopause syndrome are widely distributed and difficult to fully evaluate by a single index, we applied the Nerve-Endocrine-Immune (NEI) network as the evaluation system for the efficacy of menopause syndrome, which is closely related with menopause syndrome and can be used to reflect the whole condition of the body.This study intends to evaluate the efficacy of Qing'E pill on the treatment of menopausal syndrome based on the NEI network by the ovariectomized rats, and then understand in depth the advantage of multi-component and multi-target for the Traditional Chinese prescription by compared with the efficacy of nilestriol and Isoflavones.