肿瘤细胞主要采用"有氧糖酵解"为其快速生长提供能量与生长所需的材料（Warburg效应）。最近有研究新发现，活性氧簇（ROS）具有可调节葡萄糖代谢的新功能，但具体的分子机制未明。申请者在已有"ROS与前列腺癌"研究的基础上，近期应用miRNA芯片发现，miRNA488在前列腺癌组织中呈低表达。生物信息学及荧光素酶报告基因分析发现，miR488可直接调控6-磷酸果糖激酶-2亚型3（PFKFB3），后者在糖酵解过程中起重要作用。转录因子Ets-1在应对氧化应激中起重要作用。本项目拟通过分子与细胞学实验，探索ROS调控Ets1-miR488-PFKFB3对前列腺癌细胞糖酵解与生长的作用机制，并在动物体内及临床前列腺癌标本进行验证，为防治该病提供新的分子靶点与实验依据，有潜在的应用价值。

Cancer cells mainly prefer "aerobic glycolysis" to generate energy and biomass for proliferation.Recent studies showed that reactive oxygen species(ROS) play new roles in the regulation of glucose metabolism.However,the machanisms and signal pathways remains unknown.Based on our research on "ROS and Prostate cancer" , we recently had identified miR488 was downregulated in prostate cancer tissues by an miRNA array analysis. Bioinformatics analysis and luciferase reporter gene assays found that miR488 directly regulates the third subtype of 6 - phosphofructokinase -2 (PFKFB3), which plays an important role in the regulation of glycolysis.Transcription factor Ets-1 was found to play an important role in oxidative response. This project aims to investigate the molecular mechanisms of "Ets1-miR488-PFKFB3" signaling in the regulation of glycolysis and cellular proliferation by ROS.Animal study and clinical prostate sample staining will be performed to confirm the molecular and celluar study.Our goal is to provide new targets and experimental data for the prevention and therapy of prostate cancer. This study has potential clinical application value.