慢性心理应激是促发AS的关键因素之一，其机制与氧化应激诱导线粒体损伤有关。SIRT1/PGC-1α通路在抗氧化应激、促进线粒体合成方面具有重要作用。研究发现Lon蛋白影响着线粒体生物合成，可能与SIRT1/PGC-1α通路存在crosstalk。课题组前期研究发现加味大柴胡汤可改善焦虑抑郁状态，具有抗氧化、抗AS作用，但机制尚未明了。本课题通过观察小鼠应激前后激素水平、线粒体功能，检测SIRT1/PGC-1α、Lon蛋白通路相关因子的表达，探讨加味大柴胡汤对慢性心理应激线粒体损伤及信号通路的干预作用；通过调控SIRT1观察其下游信号因子和Lon蛋白通路的表达，验证其存在crosstalk；运用siRNA技术验证加味大柴胡汤抗线粒体损伤的作用靶点，阐明其调控两条通路crosstalk的机制。本课题研究结果可为本方从“气血失和”论治AS提供佐证，为开拓中医药防治AS提供新的思路和方法。

Chronic psychological stress is one of the key factors to promote AS, and its mechanism is related to oxidative stress induced mitochondrial damage. The SIRT1/PGC-1α pathway plays an important role in antioxidative stress and promotion of mitochondrial synthesis.Study found that Lon protein affects mitochondrial biosynthesis and may have crosstalk with the SIRT1/PGC-1α pathway. The previous study of the research group found that modified Dachaihu Decoction can improve anxiety and depression, and had anti-oxidation and anti-AS effects, but the mechanism is not yet clear. In this study, hormone levels and mitochondrial function will measure before and after stress in mice,and examining the expression of SIRT1/PGC-1α and Lon protein signaling pathway-related factors, to investigate the effect of Modified DaChaiHu Decoction on the mitochondrial injury and signal pathway by chronic psychological stress.By regulating SIRT1,the downstream signaling factors and Lon protein pathways were observed to verify the existence of crosstalk.Using siRNA technology to verify the target of modified DaChaihu Decoction against mitochondrial damage, to explore its mechanism for regulating crosstalk on both channels. The research results of this project can provide evidence for the treatment of AS from modified DaChaihu Decoction from the theory of "qi and blood loss", and provide new ideas and methods for the development of prevention and treatment of AS in traditional Chinese medicine.