基于多氯联苯厌氧代谢速率慢、非水溶性介体非均相催化效率低以及介体不能有效回收的问题，项目提出一种链锚-超分子介体生物催化剂的构建策略。拟①通过环糊精包结介体，制备环糊精介体包合物，构筑非水溶性介体的拟均相生物催化剂，提高非水溶性介体的催化效能；通过磁性Fe3O4纳米粒子固定环糊精介体包合物，制备链锚-超分子介体生物催化剂，实现介体的有效分离与回收；②链锚-超分子介体用于催化加速PCBs的厌氧脱氯过程，验证其催化效能，解析生物催化机理，完善胞外介体呼吸电子传递链体系，构建介体调控电子传递构-效模型；③研发与链锚-超分子介体生物催化剂相适配的生物反应器系统，实现介体的高效催化和有效回收；同时考察相关参数对多氯联苯生物转化效率及中间产物生成的影响，建立介体催化-生物降解高效协同调控策略，形成新的厌氧生物强化技术系统，并拓展其在处理实际废水中的应用。

The project carries out to overcome the drawbacks of slow anaerobic biotransformation rate of polychlorinated biphenyls (PCBs), heterogeneous catalysis of redox mediator (RM) and loss of RM as water flow. Chain anchor supramolecular mediator (CA-SM) biological catalysts with a high catalytic efficiency and easy recovery is to be prepared. Firstly, cyclodextrin (CD) clathrates RM molecules to obtain the CD-RM inclusions. Then, CD-RM inclusions are immobilized to magnetic nanoparticles, producing CA-SM biological catalysts. Due to the separation of magnetic nanoparticles in the external magnetic field, the CA-SM biological catalysts can easily separete and reuse. The CA-SM biological catalysts employ for catalyzing anaerobic dechloridation of PCBs. Catalytic mechanism is analysised and the extracellular mediators of the respiratory electron transport chain system constructed. Last but not the least, bioreactor system matching with CA-SM biological catalysts will develop to achieve RM’s effective recovery and biotransformation of PCBs. At the same time, the influence of the relevant parameters and the intermediate product during PCBs biological conversion are studied. The strategy of efficient biodegradatation for PCBs and new technology is to be established to strengthen anaerobic system, which will provide reference for the evolvement and promoting further utilizations in practical wastewater treatment process.