基于肠道菌群的人参、黄连“药对”治疗2型糖尿病的抗炎机制研究

人参、黄连“药对”为中医治疗2型糖尿病的常用对药，前期研究证实人参、黄连“药对”能够改善胰岛素抵抗，调节糖代谢，机制可能与抗炎作用有关，但其抗炎机制不明确。目前，2型糖尿病的低度炎症状态与肠道菌群紊乱相关已经得到证实。因此我们提出假说：人参、黄连“药对”通过调节肠道菌群，纠正因菌群紊乱导致的内毒素吸收增加以及肠道短链脂肪酸成分改变，起到抗炎作用，从而能够调节血糖。为验证此假说，本研究将以二甲双胍为对照，观察人参、黄连“药对”对db/db小鼠血糖、空腹胰岛素以及炎症因子的影响，同时采用宏基因组测序技术对小鼠进行全基因组测序，采用气相色谱法测定小鼠肠道短链脂肪酸，采用酶联免疫法测定血清LPS、Zonulin、LPS等的水平，蛋白印迹法测定肠道组织Occludin表达水平。最终，从调节肠道菌群的角度，明确人参、黄连“药对”抗炎、改善胰岛素抵抗的机制，这将为中医药防治糖尿病提供重要的科学依据。

The drug pair of ginseng and rhizoma coptidis is often used to treat type 2 diabetes. The preliminary studies have suggested that the ginseng and rhizoma coptidis drug pair can improve insulin resistance and regulate glucose metabolism, which may be related to its anti-inflammatory effect . But the underlying mechanism is unclear. Moreover, it has been proved that the low-grade inflammation state of type 2 diabetes has close relationship with intestinal flora disorder. Therefore we propose a hypothesis that the anti-inflammation and glucose regulation effect of ginseng and rhizoma coptidis drug pair may act through regulating the intestinal flora, correcting the increased absorption of endotoxin and changing the composition of intestinal short chain fatty acids. In order to test this hypothesis, this study will choose metformin as contrast to observe the impact of ginseng and rhizoma coptidis drug pair on blood glucose, fasting insulin and inflammatory factors. Meanwhile, the whole genome of intestinal flora will be detected by macro genome sequencing technology and mice intestinal fatty acids will be detected using gas chromatography. We also measure levels of serum LPS, Zonulin and LPS by enzyme-linked immunoassay, and detect intestinal tissue Occludin expression level with the protein imprinting method. The db/db mice will be used in this study. In the end, the result is expected to uncover the anti-inflammatory mechanism of ginseng and rhizoma coptidis drug pair based on intestinal flora. This study will provide important scientific basis for prevention and treatment of Traditional Chinese medicine for diabetes mellitus.