黑柿成熟时果皮呈黑色，营养价值较高，是我国稀有且独特的资源。同时黑柿有果皮颜色黑黄相间的嵌合体果实，具很高的研究和开发应用价值。目前其黑色果皮形成的机理并不清楚，解析这一形成机制可能是对现有色素研究的重要补充和完善，同时为嵌合体研究提供新的理论依据。本项目团队前期研究发现，黑柿黑色果皮由花青苷累积产生，并找到与黑柿遗传背景相似性>95%但果皮呈黄色的红柿。本项目拟以转色关键时期的黑柿、红柿和嵌合体果实为试材，比较果皮组织解剖学及生理指标差异，确定这些因素是否影响黑柿果皮黑色产生。利用RNA-seq技术挖掘与黑柿花青苷累积相关的关键基因，并在前期创建的黑柿杂交后代群体中结合关键基因特异标记与果皮色泽表型分析确认关键基因并验证其功能。本项目的实施，不仅有助于解析黑柿色素累积的分子机理，而且为以嵌合体为途径的种质资源创新提供新的理论依据，同时为柿色泽改良和育种提供优良候选基因，具有重要应用价值。

Black persimmon’s peel is black when mature, with high nutritional value, which is a rare and unique resource in china. At the same time, there are black persimmon chimera fruit whose peel color is yellow and black, which make it have a high value of research and application. At present, the mechanism of the black peel formation was not clear. It may be an important supplement and improvement to the existing pigment research, and provide a new theoretical basis for the study of the chimera. In our preliminary study we found that, the black color of black persimmon’s peel was caused by anthocyanin accumulation. And we found a cultivar named ‘red persimmon’ whose genetic similarity was 95% to black persimmon, but its peel color was yellow. The project will pick up fruits of black persimmon, red persimmon and the chimera during their key period of color changing, compare the differences in pericarp tissue anatomy and physiological indexes, and determine if these factors affect black persimmon’s black peel formation. RNA-seq technology was also used to mine key genes related to black persimmon anthocyanin accumulation. Key genes were verified and functionality identified in the black persimmon progenies primarily created, combined with key gene specific markers and progenies’ peel color phenotype. The implementation of the project was not only helpful to elucidate molecular mechanism of black persimmon pigment accumulation, but also provide new theoretical basis for resources innovation by using the chimera germplasm. At the same time, the project will provide excellent candidate genes for persimmon color improvement and breeding, which is of great application value.