MOV10是宿主细胞内重要的抗HIV-1的蛋白因子。近年来，MOV10与HIV-1相互作用的分子机理已成为HIV-1领域的研究热点。我们前期的研究发现：MOV10可抑制HIV-1 Vif对APOBEC3G（A3G）的降解，意味着MOV10可增强抗病毒蛋白A3G拮抗HIV-1的活性；此外，HIV-1 Rev可通过与MOV10的结合促进Rev/RRE依赖性基因的表达；HIV-1还可通过某种未知机制抑制MOV10的表达。本项目拟对上述分子机理进行深入研究，探索MOV10与A3G二者抗HIV-1活性的相关性，完善人们对MOV10抗HIV-1功能的认识；此外，还将从Rev与MOV10的相互作用以及HIV-1抑制MOV10表达这两个现象入手阐明HIV-1拮抗MOV10的分子机理。本项目的研究结果将为HIV-1与宿主相互作用的分子机理提供更多的理论依据，也对研发防控HIV-1感染的新方法产生重要的影响。

The host cell protein MOV10 is one of the most important anti-HIV-1factors and the molecular mechanism of the interaction between MOV10 and HIV-1 has recently become a hot topic in HIV-1 research. Our preliminary work demonstrated that MOV10 can inhibit the degradation of antiviral protein APOBEC3G (A3G) by interfering Vif-A3G degradation pathway, suggesting that MOV10 may enhance the anti-HIV-1 activity of A3G; moreover, HIV-1 Rev enhances the expression of Rev/RRE-dependent gene by interacting with MOV10; HIV-1 is able to suppress MOV10 protein expression through an unknown mechanism. Our overall goal is to make better understand of the above mechanism by intensive studies. The specific aims of the proposed research are: 1) To explore the correlation of anti-HIV-1 activity between MOV10 and A3G and improve the understanding of the anti-HIV-1 function of MOV10; 2) To clarify the molecular bases of how HIV-1 counteracts MOV10 by investigating the mechanism of the interaction between HIV-1 Rev and MOV10 and to reveal the unknown mechanism of how HIV-1 inhibits MOV10 expression. The results of these studies will provide more experimental and theoretical evidences for the molecular mechanism of the interaction between HIV-1 and host cells, and will contribute important new information to the development of new methods of HIV-1 prevention and treatment.