每年的人(禽)流感频发给我国的公共卫生带来了严重的威胁。目前，缺乏高效低毒的流感粘膜疫苗应对人(禽)流感病毒抗原频繁变异。粘膜佐剂的有效性、安全性及其作用机理是粘膜疫苗研究中的关键问题。我们的前期研究已经证明芳香辟秽中药-冰香散挥发油滴鼻或者雾化给药具有显著的诱导和增强粘膜免疫的活性，且对流感病毒有良好的预防作用和直接抑制作用。本申请项目在此基础上，进一步研究芳香辟秽中药作为流感疫苗粘膜佐剂的活性。本研究为筛选出安全有效的流感粘膜佐剂疫苗奠定坚实的基础，对于提高中医药预防流感的水平和我国的流感防治工作有着积极的现实意义。

Frequent occurrence of human avian influenza has posed a serious threat to public health in China every year. There has been no safe and effective mucosa vaccine for frequently antigenic variation of flu virus so far. Therefore, researching and developing effectiveness, safety and action mechanism of mucosal adjuvant are key question to mucosa vaccine. Our previous study indicated that the volatile oil of Bingxiang powder had a marked inductive effect on the mucosal immunity, also direct inhibitory and preventive effect on influenza virus by intranasal and aerosol treatment. Then, the project make further study on the activity of aromatic Chinese herbs as a flu vaccine mucosal adjuvant. We hypothesized that the activity of aromatic Chinese herbs as a flu vaccine mucosal adjuvant is closely related to nasal mucosal immunity. The project intends to further develop the regulation approaches of aromatic Chinese herbs as a flu vaccine mucosal adjuvant at the cellular and molecular levels, based on follicular helper T cells (Tfh) on nasal-associated lymphoid tissue (NALT), specific antibody-secreting cells, Toll receptor and defensin on nasal mucosa, and the dendritic cell phenotype and function, etc. This research will provide a solid foundation for safe and effective influenza virus mucosal vaccine, and improve TCM level in controlling and preventing influenza. It has a positive and practical significance to the job of influenza prevention and cure in China.